Increased IL-17-expressing γδ T cells in seborrhoeic dermatitis-like lesions of the Mpzl3 knockout mice

Tongyu Wikramanayake, Penelope Hirt, Maram Almastadi, Hunter Mitchell, Marjana Tomic-Canic, Laura Romero, Denisse Garcia, Natasa Strbo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Seborrhoeic Dermatitis (SD) is a common inflammatory skin disorder, but its molecular pathogenesis remains elusive. Previously, we have established the Mpzl3 knockout (−/−) mice as a model for SD. In this study, we focused on early phases of skin inflammation and determined the cytokine profiles and identified immune cell types in the lesional skin in the Mpzl3 −/− mice. Using flow cytometry, we detected a significant increase of CD45+ leucocytes, CD3+ T lymphocytes and especially γδ T cells but not αβ T cells in the lesional skin compared to control. We also detected high levels of IL-17 and determined that the γδ T cells were a major contributing source. CD3+ and γδ T cell localization in the skin was verified by indirect immunofluorescent staining. Since neither γδ T cells nor IL-17 had been implicated in SD, our study provides novel insights into the role of MPZL3 in the pathogenesis of SD-like skin inflammation.

Original languageEnglish (US)
Pages (from-to)1408-1411
Number of pages4
JournalExperimental Dermatology
Volume27
Issue number12
DOIs
StatePublished - Dec 1 2018

Fingerprint

Dermatitis
T-cells
Interleukin-17
Knockout Mice
Skin
T-Lymphocytes
Inflammation
Flow cytometry
Flow Cytometry
Leukocytes
Staining and Labeling
Cytokines

Keywords

  • gamma delta T cells
  • IL-17
  • inflammation
  • seborrhoeic dermatitis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Increased IL-17-expressing γδ T cells in seborrhoeic dermatitis-like lesions of the Mpzl3 knockout mice. / Wikramanayake, Tongyu; Hirt, Penelope; Almastadi, Maram; Mitchell, Hunter; Tomic-Canic, Marjana; Romero, Laura; Garcia, Denisse; Strbo, Natasa.

In: Experimental Dermatology, Vol. 27, No. 12, 01.12.2018, p. 1408-1411.

Research output: Contribution to journalArticle

Wikramanayake, Tongyu ; Hirt, Penelope ; Almastadi, Maram ; Mitchell, Hunter ; Tomic-Canic, Marjana ; Romero, Laura ; Garcia, Denisse ; Strbo, Natasa. / Increased IL-17-expressing γδ T cells in seborrhoeic dermatitis-like lesions of the Mpzl3 knockout mice. In: Experimental Dermatology. 2018 ; Vol. 27, No. 12. pp. 1408-1411.
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