Increased growth capacity of cervical-carcinoma cells over-expressing manganous superoxide dismutase

Bernardetta Palazzotti, Giovanni Pani, Renata Colavitti, Maria Emilia De Leo, Barbara Bedogni, Silvia Borrello, Tommaso Galeotti

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Increases in the expression of manganese-dependent superoxide dismutase (MnSOD) have been detected in several classes of human and experimental tumors and appear to correlate with poorer prognosis in human neuro- epithelial, ovarian and cervical tumors. To delineate the relevance of MnSOD expression to tumor-cell growth and survival, a human MnSOD cDNA was over- expressed in the HeLa cervical-carcinoma cell line. MnSOD over-expression had marginal effects on the growth of HeLa cells in standard medium, but markedly protected the cells from growth suppression and cell death in conditions of serum deprivation. Serum starvation did not affect expression of endogenous MnSOD in wild-type HeLa cells, but was associated with increases in cell death and in the generation of intracellular oxygen radicals. By contrast, in HT29 colon-carcinoma cells, which are relatively resistant to growth-factor withdrawal, serum deprivation was associated with increases in MnSOD expression and activity. Together these observations suggest that MnSOD provides a mechanism for counteracting the intracellular oxidative processes that impair cell growth and viability in the context of growth-factor withdrawal and, in this context, may promote tumor-cell survival in vivo in conditions normally unfavorable to cell growth.

Original languageEnglish (US)
Pages (from-to)145-150
Number of pages6
JournalInternational Journal of Cancer
Volume82
Issue number1
DOIs
StatePublished - Jul 2 1999

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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