Increased glucose transporter-1 EXPRESSION on intermediate monocytes from HIV-infected women with subclinical cardiovascular disease

Tiffany R. Butterfield, David B. Hanna, Robert C. Kaplan, Jorge R. Kizer, Helen G. Durkin, Mary A. Young, Marek J. Nowicki, Phyllis C. Tien, Elizabeth T. Golub, Michelle A. Floris-Moore, Kehmia Titanji, Margaret A Fischl, Sonya L. Heath, Jefferey Martinson, Suzanne M. Crowe, Clovis S. Palmer, Alan L. Landay, Joshua J. Anzinger

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

OBJECTIVE:: People living with HIV (PLWH) have chronic immune activation and increased cardiovascular disease (CVD) risk. Activation of monocytes and T lymphocytes causes up-regulation of glucose transporter-1 (GLUT1) for efficient function. PLWH have an increased percentage of GLUT1-expressing monocytes and T lymphocytes, but it is unclear if these cells are associated with CVD. We evaluated expression of GLUT1 and CD38 on monocyte and T lymphocyte populations from HIV-infected women with subclinical CVD. METHODS:: Participants with >75 percentile (n=15) and <25 percentile (n?=?15) age-adjusted intima-media thickness (IMT) at the right common carotid artery and bifurcation were identified from the Womenʼs Interagency HIV Study. Groups were matched by age, race/ethnicity, smoking status, and CD4 count. All women were receiving suppressive antiretroviral therapy except for one high and one low IMT participant. Monocyte and T lymphocyte populations were evaluated for GLUT1 and CD38 expression using flow cytometry. RESULTS:: Intermediate monocytes from high IMT women had significantly increased expression of GLUT1 (310 MFI vs. 210 MFI, p?=?0.024) (66.4% vs. 48.5%, p?=?0.031) and CD38 (339 MFI vs. 211 MFI, p?=?0.002) (10.5% vs. 3.8%, p?=?0.0002) compared to women with low IMT. High and low IMT participants showed no differences in GLUT1 or CD38 expression on classical monocytes, non-classical monocytes, CD4+ and CD8+ T lymphocytes. CONCLUSION:: GLUT1-expressing intermediate monocytes are elevated in HIV-infected women with subclinical CVD. These cells may contribute to development of CVD in PLWH and could be a novel target to limit inflammation.

Original languageEnglish (US)
JournalAIDS
DOIs
StateAccepted/In press - Nov 9 2016

    Fingerprint

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Butterfield, T. R., Hanna, D. B., Kaplan, R. C., Kizer, J. R., Durkin, H. G., Young, M. A., Nowicki, M. J., Tien, P. C., Golub, E. T., Floris-Moore, M. A., Titanji, K., Fischl, M. A., Heath, S. L., Martinson, J., Crowe, S. M., Palmer, C. S., Landay, A. L., & Anzinger, J. J. (Accepted/In press). Increased glucose transporter-1 EXPRESSION on intermediate monocytes from HIV-infected women with subclinical cardiovascular disease. AIDS. https://doi.org/10.1097/QAD.0000000000001320