Increased expression of iron-regulating genes in monkey and human glaucoma

Ronald H. Farkas, Itay Chowers, Abigail S Hackam, Masaki Kageyama, Robert W. Nickells, Deborah C. Otteson, Elia J. Duh, Chenwei Wang, Danielle F. Valenta, Tushara L. Gunatilaka, Mary E. Pease, Harry A. Quigley, Donald J. Zack

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

PURPOSE. To understand the mechanisms mediating retinal ganglion cell loss in glaucoma, the gene expression patterns were compared for transferrin, ceruloplasmin, and ferritin between normal and glaucomatous retina in monkey and human eyes. METHODS. Laser photocoagulation was used to produce unilateral experimental glaucoma in monkeys. Gene expression was assessed by in situ hybridization and quantitative reverse transcription polymerase chain reaction (PCR). Immunohistochemistry was used to examine the retinal expression of iron-related proteins in the retina in experimental monkey glaucoma and human glaucoma. RESULTS. Comparison of glaucomatous with control monkey retinas demonstrated increased mRNA expression of transferrin, ceruloplasmin, and ferritin heavy and light chains. In situ hybridization localized retinal gene expression of transferrin mainly to the inner nuclear layer and ferritin to both the inner and outer nuclear layers. Immunohistochemical examination of monkey and human glaucoma for these iron-related proteins demonstrated increases at the protein level. CONCLUSIONS. Increased mRNA and protein levels of the iron-regulating proteins transferrin, ceruloplasmin, and ferritin are present in glaucoma. Together, these results suggest the involvement of iron and copper metabolism and associated antioxidant systems in the pathogenesis of glaucoma.

Original languageEnglish
Pages (from-to)1410-1417
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume45
Issue number5
DOIs
StatePublished - May 1 2004
Externally publishedYes

Fingerprint

Glaucoma
Haplorhini
Iron
Transferrin
Ceruloplasmin
Genes
Ferritins
Apoferritins
Retina
Proteins
Gene Expression
In Situ Hybridization
Messenger RNA
Retinal Ganglion Cells
Light Coagulation
Reverse Transcription
Copper
Lasers
Antioxidants
Immunohistochemistry

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Farkas, R. H., Chowers, I., Hackam, A. S., Kageyama, M., Nickells, R. W., Otteson, D. C., ... Zack, D. J. (2004). Increased expression of iron-regulating genes in monkey and human glaucoma. Investigative Ophthalmology and Visual Science, 45(5), 1410-1417. https://doi.org/10.1167/iovs.03-0872

Increased expression of iron-regulating genes in monkey and human glaucoma. / Farkas, Ronald H.; Chowers, Itay; Hackam, Abigail S; Kageyama, Masaki; Nickells, Robert W.; Otteson, Deborah C.; Duh, Elia J.; Wang, Chenwei; Valenta, Danielle F.; Gunatilaka, Tushara L.; Pease, Mary E.; Quigley, Harry A.; Zack, Donald J.

In: Investigative Ophthalmology and Visual Science, Vol. 45, No. 5, 01.05.2004, p. 1410-1417.

Research output: Contribution to journalArticle

Farkas, RH, Chowers, I, Hackam, AS, Kageyama, M, Nickells, RW, Otteson, DC, Duh, EJ, Wang, C, Valenta, DF, Gunatilaka, TL, Pease, ME, Quigley, HA & Zack, DJ 2004, 'Increased expression of iron-regulating genes in monkey and human glaucoma', Investigative Ophthalmology and Visual Science, vol. 45, no. 5, pp. 1410-1417. https://doi.org/10.1167/iovs.03-0872
Farkas, Ronald H. ; Chowers, Itay ; Hackam, Abigail S ; Kageyama, Masaki ; Nickells, Robert W. ; Otteson, Deborah C. ; Duh, Elia J. ; Wang, Chenwei ; Valenta, Danielle F. ; Gunatilaka, Tushara L. ; Pease, Mary E. ; Quigley, Harry A. ; Zack, Donald J. / Increased expression of iron-regulating genes in monkey and human glaucoma. In: Investigative Ophthalmology and Visual Science. 2004 ; Vol. 45, No. 5. pp. 1410-1417.
@article{3c90b908a1824e66b035f74e87551e83,
title = "Increased expression of iron-regulating genes in monkey and human glaucoma",
abstract = "PURPOSE. To understand the mechanisms mediating retinal ganglion cell loss in glaucoma, the gene expression patterns were compared for transferrin, ceruloplasmin, and ferritin between normal and glaucomatous retina in monkey and human eyes. METHODS. Laser photocoagulation was used to produce unilateral experimental glaucoma in monkeys. Gene expression was assessed by in situ hybridization and quantitative reverse transcription polymerase chain reaction (PCR). Immunohistochemistry was used to examine the retinal expression of iron-related proteins in the retina in experimental monkey glaucoma and human glaucoma. RESULTS. Comparison of glaucomatous with control monkey retinas demonstrated increased mRNA expression of transferrin, ceruloplasmin, and ferritin heavy and light chains. In situ hybridization localized retinal gene expression of transferrin mainly to the inner nuclear layer and ferritin to both the inner and outer nuclear layers. Immunohistochemical examination of monkey and human glaucoma for these iron-related proteins demonstrated increases at the protein level. CONCLUSIONS. Increased mRNA and protein levels of the iron-regulating proteins transferrin, ceruloplasmin, and ferritin are present in glaucoma. Together, these results suggest the involvement of iron and copper metabolism and associated antioxidant systems in the pathogenesis of glaucoma.",
author = "Farkas, {Ronald H.} and Itay Chowers and Hackam, {Abigail S} and Masaki Kageyama and Nickells, {Robert W.} and Otteson, {Deborah C.} and Duh, {Elia J.} and Chenwei Wang and Valenta, {Danielle F.} and Gunatilaka, {Tushara L.} and Pease, {Mary E.} and Quigley, {Harry A.} and Zack, {Donald J.}",
year = "2004",
month = "5",
day = "1",
doi = "10.1167/iovs.03-0872",
language = "English",
volume = "45",
pages = "1410--1417",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "5",

}

TY - JOUR

T1 - Increased expression of iron-regulating genes in monkey and human glaucoma

AU - Farkas, Ronald H.

AU - Chowers, Itay

AU - Hackam, Abigail S

AU - Kageyama, Masaki

AU - Nickells, Robert W.

AU - Otteson, Deborah C.

AU - Duh, Elia J.

AU - Wang, Chenwei

AU - Valenta, Danielle F.

AU - Gunatilaka, Tushara L.

AU - Pease, Mary E.

AU - Quigley, Harry A.

AU - Zack, Donald J.

PY - 2004/5/1

Y1 - 2004/5/1

N2 - PURPOSE. To understand the mechanisms mediating retinal ganglion cell loss in glaucoma, the gene expression patterns were compared for transferrin, ceruloplasmin, and ferritin between normal and glaucomatous retina in monkey and human eyes. METHODS. Laser photocoagulation was used to produce unilateral experimental glaucoma in monkeys. Gene expression was assessed by in situ hybridization and quantitative reverse transcription polymerase chain reaction (PCR). Immunohistochemistry was used to examine the retinal expression of iron-related proteins in the retina in experimental monkey glaucoma and human glaucoma. RESULTS. Comparison of glaucomatous with control monkey retinas demonstrated increased mRNA expression of transferrin, ceruloplasmin, and ferritin heavy and light chains. In situ hybridization localized retinal gene expression of transferrin mainly to the inner nuclear layer and ferritin to both the inner and outer nuclear layers. Immunohistochemical examination of monkey and human glaucoma for these iron-related proteins demonstrated increases at the protein level. CONCLUSIONS. Increased mRNA and protein levels of the iron-regulating proteins transferrin, ceruloplasmin, and ferritin are present in glaucoma. Together, these results suggest the involvement of iron and copper metabolism and associated antioxidant systems in the pathogenesis of glaucoma.

AB - PURPOSE. To understand the mechanisms mediating retinal ganglion cell loss in glaucoma, the gene expression patterns were compared for transferrin, ceruloplasmin, and ferritin between normal and glaucomatous retina in monkey and human eyes. METHODS. Laser photocoagulation was used to produce unilateral experimental glaucoma in monkeys. Gene expression was assessed by in situ hybridization and quantitative reverse transcription polymerase chain reaction (PCR). Immunohistochemistry was used to examine the retinal expression of iron-related proteins in the retina in experimental monkey glaucoma and human glaucoma. RESULTS. Comparison of glaucomatous with control monkey retinas demonstrated increased mRNA expression of transferrin, ceruloplasmin, and ferritin heavy and light chains. In situ hybridization localized retinal gene expression of transferrin mainly to the inner nuclear layer and ferritin to both the inner and outer nuclear layers. Immunohistochemical examination of monkey and human glaucoma for these iron-related proteins demonstrated increases at the protein level. CONCLUSIONS. Increased mRNA and protein levels of the iron-regulating proteins transferrin, ceruloplasmin, and ferritin are present in glaucoma. Together, these results suggest the involvement of iron and copper metabolism and associated antioxidant systems in the pathogenesis of glaucoma.

UR - http://www.scopus.com/inward/record.url?scp=2442658143&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442658143&partnerID=8YFLogxK

U2 - 10.1167/iovs.03-0872

DO - 10.1167/iovs.03-0872

M3 - Article

C2 - 15111596

AN - SCOPUS:2442658143

VL - 45

SP - 1410

EP - 1417

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 5

ER -