Increased Expression of Epileptiform Spike/Wave Discharges One Year after Mild, Moderate, or Severe Fluid Percussion Brain Injury in Rats

Thomas Sick, Joseph Wasserman, Amade Bregy, Justin Sick, W. Dalton Dietrich, Helen Bramlett

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In this study, we describe increased expression of cortical epileptiform spike/wave discharges (SWD) in rats one year after mild, moderate, or severe fluid percussion traumatic brain injury (fpTBI). Groups of rats consisted of animals that had received mild, moderate, or severe fpTBI, or sham operation one year earlier than electrocorticography (ECoG) recordings. In addition, we included a group of age-matched naïve animals. ECoG was recorded from awake animals using epidural electrodes implanted on the injured hemisphere (right), sham-operated hemisphere (right), or right hemisphere in naïve animals. The SWDs were detected automatically using Fast Fourier Transformation and a novel algorithm for comparing changes in spectral power to control (nonepileptical) ECoG. The fpTBI resulted in increased expression of SWDs one year after injury compared with sham-operated or naïve animals. The number of SWD-containing ECoG epochs recorded in a 1 h recording session were: naïve 12.9 ± 10.3, n = 8, sham 23.6 ± 8.2, n = 10, mild TBI 78.9 ± 23.9, n = 10, moderate TBI 61.3 ± 32.5, n = 12, severe TBI 72.5 ± 28.3, n = 11 (mean ± standard error of the mea). Increased expression of SWDs was not related to injury severity. SWDs were observed to a lesser extent even in sham-operated and naïve animals. The data indicate that fpTBI exacerbates expression of SWDs in the rat and that this increase may be observed at least one year after injury. As others have discussed, the spontaneous occurrence of these epileptiform events in rodents limits the use of this model for investigations of acquired epilepsy, at least of the nonconvulsive type, after TBI.

Original languageEnglish (US)
Pages (from-to)2467-2474
Number of pages8
JournalJournal of Neurotrauma
Volume34
Issue number16
DOIs
StatePublished - Aug 15 2017

Fingerprint

Percussion
Brain Injuries
Wounds and Injuries
Implanted Electrodes
Rodentia
Epilepsy
Research Design
Age Groups
Electrocorticography
Traumatic Brain Injury

Keywords

  • electrocorticogram
  • electroencephalogram
  • epilepsy
  • fluid percussion
  • nonconvulsive seizures
  • spike wave discharges
  • traumatic brain injury

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Increased Expression of Epileptiform Spike/Wave Discharges One Year after Mild, Moderate, or Severe Fluid Percussion Brain Injury in Rats. / Sick, Thomas; Wasserman, Joseph; Bregy, Amade; Sick, Justin; Dalton Dietrich, W.; Bramlett, Helen.

In: Journal of Neurotrauma, Vol. 34, No. 16, 15.08.2017, p. 2467-2474.

Research output: Contribution to journalArticle

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abstract = "In this study, we describe increased expression of cortical epileptiform spike/wave discharges (SWD) in rats one year after mild, moderate, or severe fluid percussion traumatic brain injury (fpTBI). Groups of rats consisted of animals that had received mild, moderate, or severe fpTBI, or sham operation one year earlier than electrocorticography (ECoG) recordings. In addition, we included a group of age-matched na{\"i}ve animals. ECoG was recorded from awake animals using epidural electrodes implanted on the injured hemisphere (right), sham-operated hemisphere (right), or right hemisphere in na{\"i}ve animals. The SWDs were detected automatically using Fast Fourier Transformation and a novel algorithm for comparing changes in spectral power to control (nonepileptical) ECoG. The fpTBI resulted in increased expression of SWDs one year after injury compared with sham-operated or na{\"i}ve animals. The number of SWD-containing ECoG epochs recorded in a 1 h recording session were: na{\"i}ve 12.9 ± 10.3, n = 8, sham 23.6 ± 8.2, n = 10, mild TBI 78.9 ± 23.9, n = 10, moderate TBI 61.3 ± 32.5, n = 12, severe TBI 72.5 ± 28.3, n = 11 (mean ± standard error of the mea). Increased expression of SWDs was not related to injury severity. SWDs were observed to a lesser extent even in sham-operated and na{\"i}ve animals. The data indicate that fpTBI exacerbates expression of SWDs in the rat and that this increase may be observed at least one year after injury. As others have discussed, the spontaneous occurrence of these epileptiform events in rodents limits the use of this model for investigations of acquired epilepsy, at least of the nonconvulsive type, after TBI.",
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