Increased contractility and impaired relaxation of the left pulmonary artery in a rabbit model of congenital diaphragmatic hernia

Augusto Frederico Schmidt, Julio Alejandro Rojas-Moscoso, Frances Lilian Lanhellas Goncalves, Rodrigo Melo Gallindo, Fabiola Zakia Monica, Edson Antunes, Rebeca Lopes Figueira, Lourenco Sbragia

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Purpose Congenital diaphragmatic hernia (CDH) is associated with pulmonary hypertension which is often difficult to manage and a significant cause of morbidity and mortality. Our aim was to study the pulmonary artery reactivity in an animal model of CDH. Methods To investigate the reactivity of the aorta and left pulmonary artery in a rabbit model of CDH, we studied the in vitro responses to the α1-adrenoceptor agonist phenylephrine (PE) and to both the muscarinic receptor agonist (ACh) and the nitric oxide (NO) donor sodium nitroprusside (SNP). Rabbits underwent surgery at 25 days of gestation. CDH was created in one fetus per horn (n = 8). Remaining fetuses were considered controls (n = 18). At term (30 days), the lung, left pulmonary artery, and aorta were dissected. In a separate group, endothelium was mechanically removed. Results There were no differences in the contractile and relaxing responses of aorta in all groups. In left pulmonary artery, PE-induced contractions were significantly greater (p<0.05) in CDH when compared with control group. The increased responsiveness to PE in CDH group was similar to that found in pulmonary artery without endothelium. The ACh-induced pulmonary artery relaxation was markedly reduced in CDH when compared with control group (p<0.05), whereas no differences were found for SNP. Conclusion Our results show increased contractility and impairment in endothelium-dependent relaxation of pulmonary artery in CDH, mimicking an endothelial dysfunction, with preserved response to endothelium-independent mechanism.

Original languageEnglish (US)
Pages (from-to)489-494
Number of pages6
JournalPediatric Surgery International
Volume29
Issue number5
DOIs
StatePublished - May 2013
Externally publishedYes

Keywords

  • Congenital diaphragmatic hernia
  • Endothelial dysfunction
  • Left pulmonary artery
  • Rabbit
  • Vascular reactivity

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Surgery

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