Increased BDNF protein expression after ischemic or PKC epsilon preconditioning promotes electrophysiologic changes that lead to neuroprotection

Jake T. Neumann, John W. Thompson, Ami P. Raval, Charles H. Cohan, Kevin B. Koronowski, Miguel A. Perez-Pinzon

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Ischemic preconditioning (IPC) via protein kinase C epsilon (PKCε) activation induces neuroprotection against lethal ischemia. Brain-derived neurotrophic factor (BDNF) is a pro-survival signaling molecule that modulates synaptic plasticity and neurogenesis. Interestingly, BDNF mRNA expression increases after IPC. In this study, we investigated whether IPC or pharmacological preconditioning (PKCε activation) promoted BDNF-induced neuroprotection, if neuroprotection by IPC or PKCε activation altered neuronal excitability, and whether these changes were BDNF-mediated. We used both in vitro (hippocampal organotypic cultures and cortical neuronal-glial cocultures) and in vivo (acute hippocampal slices 48 hours after preconditioning) models of IPC or PKCε activation. BDNF protein expression increased 24 to 48 hours after preconditioning, where inhibition of the BDNF Trk receptors abolished neuroprotection against oxygen and glucose deprivation (OGD) in vitro. In addition, there was a significant decrease in neuronal firing frequency and increase in threshold potential 48 hours after preconditioning in vivo, where this threshold modulation was dependent on BDNF activation of Trk receptors in excitatory cortical neurons. In addition, 48 hours after PKCε activation in vivo, the onset of anoxic depolarization during OGD was significantly delayed in hippocampal slices. Overall, these results suggest that after IPC or PKCε activation, there are BDNF-dependent electrophysiologic modifications that lead to neuroprotection.

Original languageEnglish (US)
Pages (from-to)121-130
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Volume35
Issue number1
DOIs
StatePublished - Jan 10 2015

Keywords

  • cell death
  • excitotoxicity
  • hyperpolarization
  • ischemic tolerance
  • Na channels
  • neurotrophins

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Neurology

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