Increased aquaporin-4 expression in ammonia-treated cultured astrocytes.

K. V. Rama Rao, M. Chen, J. M. Simard, M. D. Norenberg

Research output: Contribution to journalArticle

Abstract

Brain edema is a serious complication of hepatic encephalopathy associated with fulminant hepatic failure (FHF). Factors responsible for such swelling are not clear, but ammonia, a toxin strongly implicated in FHF, is known to induce astrocyte swelling. The mechanism(s) responsible for ammonia-induced swelling, however, are not known. Aquaporins are plasma membrane proteins that mediate transmembrane water movement. To investigate the potential role of aquaporins in astrocyte swelling, we measured aquaporin-4 (AQP-4) protein expression in cultured astrocytes exposed to 5 mM NH4Cl. AQP-4 levels significantly increased 10 h after treatment with ammonia, and displayed a progressive rise up to 48 h, which appeared to precede the onset of astrocyte swelling. AQP-4 may be involved in the astrocyte swelling associated with hyperammonemic states.

Original languageEnglish
Pages (from-to)2379-2382
Number of pages4
JournalNeuroReport
Volume14
Issue number18
StatePublished - Dec 19 2003

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Aquaporin 4
Ammonia
Astrocytes
Aquaporins
Acute Liver Failure
Water Movements
Hepatic Encephalopathy
Brain Edema
Blood Proteins
Membrane Proteins
Cell Membrane

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Rama Rao, K. V., Chen, M., Simard, J. M., & Norenberg, M. D. (2003). Increased aquaporin-4 expression in ammonia-treated cultured astrocytes. NeuroReport, 14(18), 2379-2382.

Increased aquaporin-4 expression in ammonia-treated cultured astrocytes. / Rama Rao, K. V.; Chen, M.; Simard, J. M.; Norenberg, M. D.

In: NeuroReport, Vol. 14, No. 18, 19.12.2003, p. 2379-2382.

Research output: Contribution to journalArticle

Rama Rao, KV, Chen, M, Simard, JM & Norenberg, MD 2003, 'Increased aquaporin-4 expression in ammonia-treated cultured astrocytes.', NeuroReport, vol. 14, no. 18, pp. 2379-2382.
Rama Rao, K. V. ; Chen, M. ; Simard, J. M. ; Norenberg, M. D. / Increased aquaporin-4 expression in ammonia-treated cultured astrocytes. In: NeuroReport. 2003 ; Vol. 14, No. 18. pp. 2379-2382.
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