Increased activity of L-type Ca2+ channels exposed to serum from patients with type I diabetes

Lisa Juntti-Berggren, Olof Larsson, Patrik Rorsman, Carina Ämmälä, Krister Bokvist, Karin Wåhlander, Pierluigi Nicotera, Jeanette Dypbukt, Sten Orrenius, Anders Hallberg, Per Olof Berggren

Research output: Contribution to journalArticle

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Abstract

Type I diabetes [insulin-dependent diabetes mellitus (IDDM)] is an autoimmune disease associated with the destruction of pancreatic β cells. Serum from patients with IDDM increased L-type calcium channel activity of insulin-producing cells and of GH3 cells derived from a pituitary tumor. The subsequent increase in the concentration of free cytoplasmic Ca2+ ([Ca2+]i) was associated with DNA fragmentation typical of programmed cell death or apoptosis. These effects of the serum were prevented by adding a blocker of voltage-activated L-type Ca2+ channels. When the serum was depleted of immunoglobulin M (IgM), it no longer affected [Ca2+]i. An IgM-mediated increase in Ca2+ influx may thus be part of the autoimmune reaction associated with IDDM and contribute to the destruction of β cells in vivo.

Original languageEnglish
Pages (from-to)86-90
Number of pages5
JournalScience
Volume261
Issue number5117
StatePublished - Jul 2 1993

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Type 1 Diabetes Mellitus
Serum
Immunoglobulin M
L-Type Calcium Channels
Pituitary Neoplasms
DNA Fragmentation
Autoimmune Diseases
Cell Death
Insulin
Apoptosis

ASJC Scopus subject areas

  • General

Cite this

Juntti-Berggren, L., Larsson, O., Rorsman, P., Ämmälä, C., Bokvist, K., Wåhlander, K., ... Berggren, P. O. (1993). Increased activity of L-type Ca2+ channels exposed to serum from patients with type I diabetes. Science, 261(5117), 86-90.

Increased activity of L-type Ca2+ channels exposed to serum from patients with type I diabetes. / Juntti-Berggren, Lisa; Larsson, Olof; Rorsman, Patrik; Ämmälä, Carina; Bokvist, Krister; Wåhlander, Karin; Nicotera, Pierluigi; Dypbukt, Jeanette; Orrenius, Sten; Hallberg, Anders; Berggren, Per Olof.

In: Science, Vol. 261, No. 5117, 02.07.1993, p. 86-90.

Research output: Contribution to journalArticle

Juntti-Berggren, L, Larsson, O, Rorsman, P, Ämmälä, C, Bokvist, K, Wåhlander, K, Nicotera, P, Dypbukt, J, Orrenius, S, Hallberg, A & Berggren, PO 1993, 'Increased activity of L-type Ca2+ channels exposed to serum from patients with type I diabetes', Science, vol. 261, no. 5117, pp. 86-90.
Juntti-Berggren L, Larsson O, Rorsman P, Ämmälä C, Bokvist K, Wåhlander K et al. Increased activity of L-type Ca2+ channels exposed to serum from patients with type I diabetes. Science. 1993 Jul 2;261(5117):86-90.
Juntti-Berggren, Lisa ; Larsson, Olof ; Rorsman, Patrik ; Ämmälä, Carina ; Bokvist, Krister ; Wåhlander, Karin ; Nicotera, Pierluigi ; Dypbukt, Jeanette ; Orrenius, Sten ; Hallberg, Anders ; Berggren, Per Olof. / Increased activity of L-type Ca2+ channels exposed to serum from patients with type I diabetes. In: Science. 1993 ; Vol. 261, No. 5117. pp. 86-90.
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AU - Bokvist, Krister

AU - Wåhlander, Karin

AU - Nicotera, Pierluigi

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