Increase in translatable mRNA for mitochondrial pyruvate carboxylase during differentiation of 3T3 preadipocytes

C. William Angus, M. Daniel Lane, Philip J Rosenfeld, Thomas J. Kelly

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

During differentiation of 3T3 preadipocytes into adipocytes the activity of pyruvate carboxylase, a key lipogenic enzyme, rises about 20-fold. This increase of enzymatic activity is correlated with a comparable rise in the rate of incorporation of [35S]methionine into immunoadsorbable pyruvate carboxylase. Polyadenylated RNA, isolated from differentiated 3T3 adipocytes, directs the synthesis of pyruvate carboxylase in a messenger-dependent reticulocyte lysate translation system at a 18-fold greater rate than that isolated from undifferentiated cells. Thus, it appears that the differentiation-induced rise in the cellular level of pyruvate carboxylase results from an increased rate of carboxylase synthesis due to a rise in the level of translatable carboxylase messenger RNA.

Original languageEnglish
Pages (from-to)1216-1222
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume103
Issue number4
DOIs
StatePublished - Dec 31 1981
Externally publishedYes

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Pyruvate Carboxylase
Adipocytes
Messenger RNA
Reticulocytes
Methionine
mitochondrial messenger RNA
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Increase in translatable mRNA for mitochondrial pyruvate carboxylase during differentiation of 3T3 preadipocytes. / Angus, C. William; Lane, M. Daniel; Rosenfeld, Philip J; Kelly, Thomas J.

In: Biochemical and Biophysical Research Communications, Vol. 103, No. 4, 31.12.1981, p. 1216-1222.

Research output: Contribution to journalArticle

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