Increase in dopamine metabolites in rat brain by neurotensin

E. Widerlov, C. D. Kilts, R. B. Mailman, Charles Nemeroff, T. J. Mc Cown, A. J. Prange, G. R. Breese

Research output: Contribution to journalArticle

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Abstract

Neurotensin (NT), an endogenous tridecapeptide, is hetero-geneously distributed in the central nervous system. The present study examined the effects of physiologically and behaviorally active doses of NT (1-100 μg intracisternally) on dopamine, serotonin and their primary metabolites as well as accumulation of dopa after inhibition of dopa decarboxylase. NT was shown to increase dopa accumulation when compared with saline treatment, suggesting that dopamine synthesis was increased. In accord with this view, NT also caused a dose-dependent increase in homovanillic acid and dihydroxyphenylacetic acid, the major metabolites of dopamine, in several brain areas (striatum, olfactory tubercles, nucleus accumbens, frontal cortex and hypothalamus). Interestingly, the increase in homovanillic acid was greater than that for dihydrodroxyphenylacetic acid. In striatum an initial increase in dopamine content after 30 μg of NT was followed by an increase and a subsequent decrease of dopamine metabolites. Several other neuropeptides (Met-enkephalin, cholecystokinin-8, thyrotropin releasing hormone, substance P and d-Arg9-NT), at doses equimolar to 30 μg of NT, did not affect dopamine metabolites, whereas certain others (β-endorphin and bombesin) increased their concentration in some brain areas. Except for the highest dose of NT, measures of serotonergic function were not affected by NT or any of the other neuropeptides.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalJournal of Pharmacology and Experimental Therapeutics
Volume223
Issue number1
StatePublished - Dec 4 1982
Externally publishedYes

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Neurotensin
Dopamine
Brain
Homovanillic Acid
Dihydroxyphenylalanine
Neuropeptides
Dopa Decarboxylase
Endorphins
Bombesin
Methionine Enkephalin
Acids
Thyrotropin-Releasing Hormone
Nucleus Accumbens
Frontal Lobe
Substance P
Hypothalamus
Serotonin
Central Nervous System

ASJC Scopus subject areas

  • Pharmacology

Cite this

Widerlov, E., Kilts, C. D., Mailman, R. B., Nemeroff, C., Mc Cown, T. J., Prange, A. J., & Breese, G. R. (1982). Increase in dopamine metabolites in rat brain by neurotensin. Journal of Pharmacology and Experimental Therapeutics, 223(1), 1-6.

Increase in dopamine metabolites in rat brain by neurotensin. / Widerlov, E.; Kilts, C. D.; Mailman, R. B.; Nemeroff, Charles; Mc Cown, T. J.; Prange, A. J.; Breese, G. R.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 223, No. 1, 04.12.1982, p. 1-6.

Research output: Contribution to journalArticle

Widerlov, E, Kilts, CD, Mailman, RB, Nemeroff, C, Mc Cown, TJ, Prange, AJ & Breese, GR 1982, 'Increase in dopamine metabolites in rat brain by neurotensin', Journal of Pharmacology and Experimental Therapeutics, vol. 223, no. 1, pp. 1-6.
Widerlov E, Kilts CD, Mailman RB, Nemeroff C, Mc Cown TJ, Prange AJ et al. Increase in dopamine metabolites in rat brain by neurotensin. Journal of Pharmacology and Experimental Therapeutics. 1982 Dec 4;223(1):1-6.
Widerlov, E. ; Kilts, C. D. ; Mailman, R. B. ; Nemeroff, Charles ; Mc Cown, T. J. ; Prange, A. J. ; Breese, G. R. / Increase in dopamine metabolites in rat brain by neurotensin. In: Journal of Pharmacology and Experimental Therapeutics. 1982 ; Vol. 223, No. 1. pp. 1-6.
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