Incident infection and resistance mutation analysis of dried blood spots collected in a field study of HIV risk groups, 2007-2010

Xierong Wei, Amanda J. Smith, David W. Forrest, Gabriel A. Cardenas, Dano W. Beck, Marlene LaLota, Lisa R. Metsch, Catlainn Sionean, S. Michele Owen, Jeffrey A. Johnson

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Objective To assess the utility of cost-effective dried blood spot (DBS) field sampling for incidence and drug resistance surveillance of persons at high risk for HIV infection. Methods We evaluated DBS collected in 2007-2010 in non-clinical settings by finger-stick from HIVpositive heterosexuals at increased risk of HIV infection (n = 124), men who have sex with men (MSM, n = 110), and persons who inject drugs (PWID, n = 58). Relative proportions of recent-infection findings among risk groups were assessed at avidity index (AI) cutoffs of ≤25%, ≤30%, and ≤35%, corresponding to an infection mean duration of recency (MDR) of 220.6, 250.4, and 278.3 days, respectively. Drug resistance mutation prevalence was compared among the risk groups and avidity indices. Results HIV antibody avidity testing of all self-reported ARV-naïve persons (n = 186) resulted in 9.7%, 11.3% and 14.0% with findings within the 221, 250, and 278-day MDRs, respectively. The proportion of ARV-naïve MSM, heterosexuals, and PWID reporting only one risk category who had findings below the suggested 30% AI was 23.1%, 6.9% and 3.6% (p<0.001), respectively. MSM had the highest prevalence of drug resistance and the only cases of transmitted multi-class resistance. Among the ARV-experienced, MSM had disproportionately more recent-infection results than did heterosexuals and PWID. Conclusions The disproportionately higher recent-infection findings for MSM as compared to PWID and heterosexuals increased as the MDR window increased. Unreported ARV use might explain greater recent-infection findings and drug resistance in this MSM population. DBS demonstrated utility in expanded HIV testing; however, optimal field handling is key to accurate recent-infection estimates.

Original languageEnglish (US)
Article numbere0159266
JournalPloS one
Issue number7
StatePublished - Jul 2016

ASJC Scopus subject areas

  • General


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