In vivo upregulation of nitric oxide synthases in healthy rats

Heng Wu, Ying Jin, Jaqueline Arias, Jorge Bassuk, Arkady Uryash, Paul Kurlansky, Keith A Webster, Jose A. Adams

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Periodic acceleration (pGz), sinusoidal motion of the whole body in a head-foot direction in the spinal axis, is a novel noninvasive means for cardiopulmonary support and induction of pulsatile shear stress. pGz increases plasma nitrite levels, in vivo and in vitro. Additionally, pGz confers cardioprotection in models of ischemia reperfusion injury. We hypothesize that pGz may also confer a cardiac phenotypic change by upregulation of the expression of the various NO synthase (NOS) isoforms in vivo. pGz was applied for 1 h to awake restrained male rats at 2 frequencies (360 and 600 cpm) and acceleration (Gz) of ±3.4 m/s2. pGz did not affect arterial blood gases or electrolytes. pGz significantly increased total nitrosylated protein levels, indicating increased NO production. pGz also increased mRNA and protein levels of eNOS and nNOS, and phosphorylated eNOS in heart. pGz increased Akt phosphorylation (p-AKT), but not total Akt, or phosphorylated ERK1/2. Inducible (i) NOS levels were undetectable with or without pGz. Immunoblotting revealed the localization of nNOS, exclusively in cardiomyocyte, and pGz increased its expression. We have demonstrated that pGz changes myocardial NOS phenotypes. Such upregulation of eNOS and nNOS was still evident 24 h after pGz. Further studies are needed to understand the biochemical and biomechanical signal transduction pathway for the observed NOS phenotype changed induced by pGz.

Original languageEnglish
Pages (from-to)63-68
Number of pages6
JournalNitric Oxide - Biology and Chemistry
Volume21
Issue number1
DOIs
StatePublished - Aug 1 2009

Fingerprint

Nitric Oxide Synthase
Rats
Up-Regulation
Phenotype
Signal transduction
Phosphorylation
Nitrites
Reperfusion Injury
Immunoblotting
Cardiac Myocytes
Electrolytes
Foot
Shear stress
Signal Transduction
Protein Isoforms
Proteins
Blood
Gases
Head
Plasmas

Keywords

  • Cardioprotection
  • Nitric oxide
  • Nitric oxide synthase (NOS)
  • Periodic acceleration

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Cancer Research
  • Physiology

Cite this

Wu, H., Jin, Y., Arias, J., Bassuk, J., Uryash, A., Kurlansky, P., ... Adams, J. A. (2009). In vivo upregulation of nitric oxide synthases in healthy rats. Nitric Oxide - Biology and Chemistry, 21(1), 63-68. https://doi.org/10.1016/j.niox.2009.05.004

In vivo upregulation of nitric oxide synthases in healthy rats. / Wu, Heng; Jin, Ying; Arias, Jaqueline; Bassuk, Jorge; Uryash, Arkady; Kurlansky, Paul; Webster, Keith A; Adams, Jose A.

In: Nitric Oxide - Biology and Chemistry, Vol. 21, No. 1, 01.08.2009, p. 63-68.

Research output: Contribution to journalArticle

Wu, H, Jin, Y, Arias, J, Bassuk, J, Uryash, A, Kurlansky, P, Webster, KA & Adams, JA 2009, 'In vivo upregulation of nitric oxide synthases in healthy rats', Nitric Oxide - Biology and Chemistry, vol. 21, no. 1, pp. 63-68. https://doi.org/10.1016/j.niox.2009.05.004
Wu, Heng ; Jin, Ying ; Arias, Jaqueline ; Bassuk, Jorge ; Uryash, Arkady ; Kurlansky, Paul ; Webster, Keith A ; Adams, Jose A. / In vivo upregulation of nitric oxide synthases in healthy rats. In: Nitric Oxide - Biology and Chemistry. 2009 ; Vol. 21, No. 1. pp. 63-68.
@article{b47b177026af4cd6b100e3927bc52a2d,
title = "In vivo upregulation of nitric oxide synthases in healthy rats",
abstract = "Periodic acceleration (pGz), sinusoidal motion of the whole body in a head-foot direction in the spinal axis, is a novel noninvasive means for cardiopulmonary support and induction of pulsatile shear stress. pGz increases plasma nitrite levels, in vivo and in vitro. Additionally, pGz confers cardioprotection in models of ischemia reperfusion injury. We hypothesize that pGz may also confer a cardiac phenotypic change by upregulation of the expression of the various NO synthase (NOS) isoforms in vivo. pGz was applied for 1 h to awake restrained male rats at 2 frequencies (360 and 600 cpm) and acceleration (Gz) of ±3.4 m/s2. pGz did not affect arterial blood gases or electrolytes. pGz significantly increased total nitrosylated protein levels, indicating increased NO production. pGz also increased mRNA and protein levels of eNOS and nNOS, and phosphorylated eNOS in heart. pGz increased Akt phosphorylation (p-AKT), but not total Akt, or phosphorylated ERK1/2. Inducible (i) NOS levels were undetectable with or without pGz. Immunoblotting revealed the localization of nNOS, exclusively in cardiomyocyte, and pGz increased its expression. We have demonstrated that pGz changes myocardial NOS phenotypes. Such upregulation of eNOS and nNOS was still evident 24 h after pGz. Further studies are needed to understand the biochemical and biomechanical signal transduction pathway for the observed NOS phenotype changed induced by pGz.",
keywords = "Cardioprotection, Nitric oxide, Nitric oxide synthase (NOS), Periodic acceleration",
author = "Heng Wu and Ying Jin and Jaqueline Arias and Jorge Bassuk and Arkady Uryash and Paul Kurlansky and Webster, {Keith A} and Adams, {Jose A.}",
year = "2009",
month = "8",
day = "1",
doi = "10.1016/j.niox.2009.05.004",
language = "English",
volume = "21",
pages = "63--68",
journal = "Nitric Oxide - Biology and Chemistry",
issn = "1089-8603",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - In vivo upregulation of nitric oxide synthases in healthy rats

AU - Wu, Heng

AU - Jin, Ying

AU - Arias, Jaqueline

AU - Bassuk, Jorge

AU - Uryash, Arkady

AU - Kurlansky, Paul

AU - Webster, Keith A

AU - Adams, Jose A.

PY - 2009/8/1

Y1 - 2009/8/1

N2 - Periodic acceleration (pGz), sinusoidal motion of the whole body in a head-foot direction in the spinal axis, is a novel noninvasive means for cardiopulmonary support and induction of pulsatile shear stress. pGz increases plasma nitrite levels, in vivo and in vitro. Additionally, pGz confers cardioprotection in models of ischemia reperfusion injury. We hypothesize that pGz may also confer a cardiac phenotypic change by upregulation of the expression of the various NO synthase (NOS) isoforms in vivo. pGz was applied for 1 h to awake restrained male rats at 2 frequencies (360 and 600 cpm) and acceleration (Gz) of ±3.4 m/s2. pGz did not affect arterial blood gases or electrolytes. pGz significantly increased total nitrosylated protein levels, indicating increased NO production. pGz also increased mRNA and protein levels of eNOS and nNOS, and phosphorylated eNOS in heart. pGz increased Akt phosphorylation (p-AKT), but not total Akt, or phosphorylated ERK1/2. Inducible (i) NOS levels were undetectable with or without pGz. Immunoblotting revealed the localization of nNOS, exclusively in cardiomyocyte, and pGz increased its expression. We have demonstrated that pGz changes myocardial NOS phenotypes. Such upregulation of eNOS and nNOS was still evident 24 h after pGz. Further studies are needed to understand the biochemical and biomechanical signal transduction pathway for the observed NOS phenotype changed induced by pGz.

AB - Periodic acceleration (pGz), sinusoidal motion of the whole body in a head-foot direction in the spinal axis, is a novel noninvasive means for cardiopulmonary support and induction of pulsatile shear stress. pGz increases plasma nitrite levels, in vivo and in vitro. Additionally, pGz confers cardioprotection in models of ischemia reperfusion injury. We hypothesize that pGz may also confer a cardiac phenotypic change by upregulation of the expression of the various NO synthase (NOS) isoforms in vivo. pGz was applied for 1 h to awake restrained male rats at 2 frequencies (360 and 600 cpm) and acceleration (Gz) of ±3.4 m/s2. pGz did not affect arterial blood gases or electrolytes. pGz significantly increased total nitrosylated protein levels, indicating increased NO production. pGz also increased mRNA and protein levels of eNOS and nNOS, and phosphorylated eNOS in heart. pGz increased Akt phosphorylation (p-AKT), but not total Akt, or phosphorylated ERK1/2. Inducible (i) NOS levels were undetectable with or without pGz. Immunoblotting revealed the localization of nNOS, exclusively in cardiomyocyte, and pGz increased its expression. We have demonstrated that pGz changes myocardial NOS phenotypes. Such upregulation of eNOS and nNOS was still evident 24 h after pGz. Further studies are needed to understand the biochemical and biomechanical signal transduction pathway for the observed NOS phenotype changed induced by pGz.

KW - Cardioprotection

KW - Nitric oxide

KW - Nitric oxide synthase (NOS)

KW - Periodic acceleration

UR - http://www.scopus.com/inward/record.url?scp=67649807453&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67649807453&partnerID=8YFLogxK

U2 - 10.1016/j.niox.2009.05.004

DO - 10.1016/j.niox.2009.05.004

M3 - Article

C2 - 19481168

AN - SCOPUS:67649807453

VL - 21

SP - 63

EP - 68

JO - Nitric Oxide - Biology and Chemistry

JF - Nitric Oxide - Biology and Chemistry

SN - 1089-8603

IS - 1

ER -