In vivo phosphorylation of neurofilament proteins in the central nervous system of immature rat and rabbit

Michael P. Honchar, Mary B. Bunge, Harish C. Agrawal

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Neurofilament proteins from brain and spinal cord of immature rat (20-35 days of age) and rabbit (15-17 days of age) were prepared by an axonal flotation technique. Examination of rat filament preparations by electron microscopy revealed a preponderance of 10 nm diameter filaments that were usually loosely aggregated although some bundles of more tightly packed filaments were present as well. The neurofilament triplet proteins of the rat and rabbit central nervous system were found to be phosphorylated 24 hr after the intracerebral injection of [32P]orthophosphate when examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by fluorography. Examination of each eluted neurofilament protein from both species showed that [32P]phosphate was retained after reelectrophoresis and fluorography. Evidence is presented that the [32P] phosphate is covalently linked to the purified neurofilament proteins by phosphoester bonds.

Original languageEnglish (US)
Pages (from-to)365-372
Number of pages8
JournalNeurochemical Research
Volume7
Issue number4
DOIs
StatePublished - Apr 1982

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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