In vivo phosphorylation of neurofilament proteins in the central nervous system of immature rat and rabbit

Michael P. Honchar, Mary B Bunge, Harish C. Agrawal

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Neurofilament proteins from brain and spinal cord of immature rat (20-35 days of age) and rabbit (15-17 days of age) were prepared by an axonal flotation technique. Examination of rat filament preparations by electron microscopy revealed a preponderance of 10 nm diameter filaments that were usually loosely aggregated although some bundles of more tightly packed filaments were present as well. The neurofilament triplet proteins of the rat and rabbit central nervous system were found to be phosphorylated 24 hr after the intracerebral injection of [32P]orthophosphate when examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by fluorography. Examination of each eluted neurofilament protein from both species showed that [32P]phosphate was retained after reelectrophoresis and fluorography. Evidence is presented that the [32P] phosphate is covalently linked to the purified neurofilament proteins by phosphoester bonds.

Original languageEnglish
Pages (from-to)365-372
Number of pages8
JournalNeurochemical Research
Volume7
Issue number4
DOIs
StatePublished - Apr 1 1982
Externally publishedYes

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Neurofilament Proteins
Phosphorylation
Neurology
Rats
Photofluorography
Central Nervous System
Rabbits
Phosphates
Flotation
Electrophoresis
Sodium Dodecyl Sulfate
Electron microscopy
Polyacrylamide Gel Electrophoresis
Brain
Spinal Cord
Electron Microscopy
Injections

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

In vivo phosphorylation of neurofilament proteins in the central nervous system of immature rat and rabbit. / Honchar, Michael P.; Bunge, Mary B; Agrawal, Harish C.

In: Neurochemical Research, Vol. 7, No. 4, 01.04.1982, p. 365-372.

Research output: Contribution to journalArticle

Honchar, Michael P. ; Bunge, Mary B ; Agrawal, Harish C. / In vivo phosphorylation of neurofilament proteins in the central nervous system of immature rat and rabbit. In: Neurochemical Research. 1982 ; Vol. 7, No. 4. pp. 365-372.
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