In vivo morphine treatment synergistically increases lps-induced caspase activity in immune organs

Michael R. Olin, Sabita Roy, Thomas Molitor

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Caspases are a family of proteins important for the elimination of infected cells through the induction of apoptosis as well as the initiation of inflammatory cytokines including IL-1β and IL-18. Morphine exposure to animals and/or cells has been associated with the induction of apoptosis. The most common practices of apoptosis detection have involved removing tissues from animal or humans and the analysis of apoptosis on cells or tissues. These methods can potentially induce spontaneous apoptosis that is unrelated to the actual treatment. The objective of this study was to develop an in vivo detection method for assessing caspase activity induced both by morphine directly and by morphine combined with lipopolysaccharide (LPS)-immune activation. Mice were administered saline, morphine, LPS, or a combination of morphine and LPS. Prior to sacrifice, mice were injected with a poly-caspase-specific apoptosis detection probe to detect internal caspase activity in vivo. Results revealed that morphine alone did not directly induce caspase activity. However, morphine significantly enhanced the LPS-induced caspase activity in spleen, thymus, and bone marrow-derived immune cells. The use of a poly-caspase detection probe methodology to label caspase activity in vivo provides a powerful quantitative tool for the in vivo analysis of caspase activity.

Original languageEnglish (US)
Pages (from-to)546-552
Number of pages7
JournalJournal of NeuroImmune Pharmacology
Volume5
Issue number4
DOIs
StatePublished - Dec 1 2010
Externally publishedYes

Fingerprint

Caspases
Morphine
Apoptosis
Lipopolysaccharides
Interleukin-18
Interleukin-1
Thymus Gland
Spleen
Bone Marrow
Cytokines

Keywords

  • apoptosis
  • caspase
  • in vivo
  • morphine

ASJC Scopus subject areas

  • Pharmacology
  • Immunology and Allergy
  • Immunology
  • Neuroscience (miscellaneous)

Cite this

In vivo morphine treatment synergistically increases lps-induced caspase activity in immune organs. / Olin, Michael R.; Roy, Sabita; Molitor, Thomas.

In: Journal of NeuroImmune Pharmacology, Vol. 5, No. 4, 01.12.2010, p. 546-552.

Research output: Contribution to journalArticle

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