In vivo laser welding of collagen-based graft material to the amnion in a rabbit model of ruptured membranes

R. A. Quintero, G. A. Mendoza, M. Allen, J. Arroyo, P. W. Bornick, W. J. Morales, N. Garabelis

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Objective: To test the feasibility of laser welding of a collagen-based graft to the amnion in a rabbit model of rupture of membranes. Methods: Pregnant white New Zealand rabbits underwent a laparotomy at 23-24 days' gestation (term = 31 days). A hysterotomy was performed in a chosen sac, and the chorion was dissected off the amnion. A 5-mm excision was made in the amnion. A Biosis (Small Intestinal Submucosa, Cook Ob/Gyn, Spencer, IN, USA) disk was welded to the edges of the amnion with YAG laser energy in the experimental sacs. In the control sacs, the defect was not repaired. The animals were sacrificed and specimens obtained at 48, 96 or 144 h. Histological results were classified as follows: (1) graft not seen; (2) graft seen, not welded; (3) graft present, welded; (4) graft present, with growth. The study was approved by the Animals Investigations Committee of the University of South Florida. Results: Twelve control and 26 experimental incisions were performed in 18 animals. The amnion could not be seen in the controls. The amnion was visualized in 42.3 % (n = 11) of the experimental sacs. Positive histological evidence of graft welding was obtained in 15.4% (n = 4) of cases. In two of these cases (7.7%), amnion growth into the graft was observed after 0.5 and 96 h. Conclusions: Despite significant limitations of the model, laser welding of a collagen graft to rabbit fetal membranes can be accomplished with YAG laser energy. Cellular growth into the graft can also be obtained. Further studies using other animal models are indicated to test the feasibility of surgically repairing prematurely ruptured membranes with collagen-based graft material.

Original languageEnglish (US)
Pages (from-to)453-456
Number of pages4
JournalPrenatal and Neonatal Medicine
Issue number6
StatePublished - Dec 1 1999


  • Animal model
  • Collagen
  • Premature rupture of membranes
  • Tissue welding

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health


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