In Vivo Imaging with a Cell-Permeable Porphyrin-Based MRI Contrast Agent

Taekwan Lee; Xiao an Zhang; Shanta Dhar; Henryk Faas; Stephen J. Lippard; Alan Jasanoff

doi:10.1016/j.chembiol.2010.05.009

In Vivo Imaging with a Cell-Permeable Porphyrin-Based MRI Contrast Agent

Taekwan Lee, Xiao an Zhang, Shanta Dhar, Henryk Faas, Stephen J. Lippard, Alan Jasanoff

Research output: Contribution to journal › Article

57 Scopus citations

Abstract

Magnetic resonance imaging (MRI) with molecular probes offers the potential to monitor physiological parameters with comparatively high spatial and temporal resolution in living subjects. For detection of intracellular analytes, construction of cell-permeable imaging agents remains a challenge. Here we show that a porphyrin-based MRI molecular imaging agent, Mn-(DPA-C₂)₂-TPPS₃, effectively penetrates cells and persistently stains living brain tissue in intracranially injected rats. Chromogenicity of the probe permitted direct visualization of its distribution by histology, in addition to MRI. Distribution was concentrated in cell bodies after hippocampal infusion. Mn-(DPA-C₂)₂-TPPS₃ was designed to sense zinc ions, and contrast enhancement was more pronounced in the hippocampus, a zinc-rich brain region, than in the caudate nucleus, which contains relatively little labile Zn²⁺. Membrane permeability, optical activity, and high relaxivity of porphyrin-based contrast agents offer exceptional functionality for in vivo imaging.

Original language	English (US)
Pages (from-to)	665-673
Number of pages	9
Journal	Chemistry and Biology
Volume	17
Issue number	6
DOIs	https://doi.org/10.1016/j.chembiol.2010.05.009
State	Published - Jun 25 2010
Externally published	Yes

Fingerprint

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

Cite this

Lee, T., Zhang, X. A., Dhar, S., Faas, H., Lippard, S. J., & Jasanoff, A. (2010). In Vivo Imaging with a Cell-Permeable Porphyrin-Based MRI Contrast Agent. Chemistry and Biology, 17(6), 665-673. https://doi.org/10.1016/j.chembiol.2010.05.009

@article{766c93bc17944d5db967cf95d10e8883,

title = "In Vivo Imaging with a Cell-Permeable Porphyrin-Based MRI Contrast Agent",

abstract = "Magnetic resonance imaging (MRI) with molecular probes offers the potential to monitor physiological parameters with comparatively high spatial and temporal resolution in living subjects. For detection of intracellular analytes, construction of cell-permeable imaging agents remains a challenge. Here we show that a porphyrin-based MRI molecular imaging agent, Mn-(DPA-C2)2-TPPS3, effectively penetrates cells and persistently stains living brain tissue in intracranially injected rats. Chromogenicity of the probe permitted direct visualization of its distribution by histology, in addition to MRI. Distribution was concentrated in cell bodies after hippocampal infusion. Mn-(DPA-C2)2-TPPS3 was designed to sense zinc ions, and contrast enhancement was more pronounced in the hippocampus, a zinc-rich brain region, than in the caudate nucleus, which contains relatively little labile Zn2+. Membrane permeability, optical activity, and high relaxivity of porphyrin-based contrast agents offer exceptional functionality for in vivo imaging.",

author = "Taekwan Lee and Zhang, {Xiao an} and Shanta Dhar and Henryk Faas and Lippard, {Stephen J.} and Alan Jasanoff",

year = "2010",

month = jun

day = "25",

doi = "10.1016/j.chembiol.2010.05.009",

language = "English (US)",

volume = "17",

pages = "665--673",

journal = "Cell Chemical Biology",

issn = "2451-9448",

publisher = "Elsevier Inc.",

number = "6",

}

TY - JOUR

T1 - In Vivo Imaging with a Cell-Permeable Porphyrin-Based MRI Contrast Agent

AU - Lee, Taekwan

AU - Zhang, Xiao an

AU - Dhar, Shanta

AU - Faas, Henryk

AU - Lippard, Stephen J.

AU - Jasanoff, Alan

PY - 2010/6/25

Y1 - 2010/6/25

N2 - Magnetic resonance imaging (MRI) with molecular probes offers the potential to monitor physiological parameters with comparatively high spatial and temporal resolution in living subjects. For detection of intracellular analytes, construction of cell-permeable imaging agents remains a challenge. Here we show that a porphyrin-based MRI molecular imaging agent, Mn-(DPA-C2)2-TPPS3, effectively penetrates cells and persistently stains living brain tissue in intracranially injected rats. Chromogenicity of the probe permitted direct visualization of its distribution by histology, in addition to MRI. Distribution was concentrated in cell bodies after hippocampal infusion. Mn-(DPA-C2)2-TPPS3 was designed to sense zinc ions, and contrast enhancement was more pronounced in the hippocampus, a zinc-rich brain region, than in the caudate nucleus, which contains relatively little labile Zn2+. Membrane permeability, optical activity, and high relaxivity of porphyrin-based contrast agents offer exceptional functionality for in vivo imaging.

AB - Magnetic resonance imaging (MRI) with molecular probes offers the potential to monitor physiological parameters with comparatively high spatial and temporal resolution in living subjects. For detection of intracellular analytes, construction of cell-permeable imaging agents remains a challenge. Here we show that a porphyrin-based MRI molecular imaging agent, Mn-(DPA-C2)2-TPPS3, effectively penetrates cells and persistently stains living brain tissue in intracranially injected rats. Chromogenicity of the probe permitted direct visualization of its distribution by histology, in addition to MRI. Distribution was concentrated in cell bodies after hippocampal infusion. Mn-(DPA-C2)2-TPPS3 was designed to sense zinc ions, and contrast enhancement was more pronounced in the hippocampus, a zinc-rich brain region, than in the caudate nucleus, which contains relatively little labile Zn2+. Membrane permeability, optical activity, and high relaxivity of porphyrin-based contrast agents offer exceptional functionality for in vivo imaging.

UR - http://www.scopus.com/inward/record.url?scp=77953724534&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953724534&partnerID=8YFLogxK

U2 - 10.1016/j.chembiol.2010.05.009

DO - 10.1016/j.chembiol.2010.05.009

M3 - Article

C2 - 20609416

AN - SCOPUS:77953724534

VL - 17

SP - 665

EP - 673

JO - Cell Chemical Biology

JF - Cell Chemical Biology

SN - 2451-9448

IS - 6

ER -

Access to Document

10.1016/j.chembiol.2010.05.009