In Vivo Imaging of Tumor-Propagating Cells, Regional Tumor Heterogeneity, and Dynamic Cell Movements in Embryonal Rhabdomyosarcoma

Myron S. Ignatius, Eleanor Chen, Natalie M. Elpek, Adam Z. Fuller, Inês M. Tenente, Ryan Clagg, Sali Liu, Jessica S. Blackburn, Corinne M. Linardic, Andrew Rosenberg, Petur G. Nielsen, Thorsten R. Mempel, David M. Langenau

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Embryonal rhabdomyosarcoma (ERMS) is an aggressive pediatric sarcoma of muscle. Here, we show that ERMS-propagating potential is confined to myf5+ cells and can be visualized in live, fluorescent transgenic zebrafish. During early tumor growth, myf5+ ERMS cells reside adjacent normal muscle fibers. By late-stage ERMS, myf5+ cells are reorganized into distinct regions separated from differentiated tumor cells. Time-lapse imaging of late-stage ERMS revealed that myf5+ cells populate newly formed tumor only after seeding by highly migratory myogenin+ ERMS cells. Moreover, myogenin+ ERMS cells can enter the vasculature, whereas myf5+ ERMS-propagating cells do not. Our data suggest that non-tumor-propagating cells likely have important supportive roles in cancer progression and facilitate metastasis.

Original languageEnglish
Pages (from-to)680-693
Number of pages14
JournalCancer Cell
Volume21
Issue number5
DOIs
StatePublished - May 25 2012
Externally publishedYes

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Embryonal Rhabdomyosarcoma
Cell Movement
Neoplasms
Myogenin
Time-Lapse Imaging
Muscles
Zebrafish
Sarcoma
Pediatrics
Neoplasm Metastasis

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Oncology

Cite this

Ignatius, M. S., Chen, E., Elpek, N. M., Fuller, A. Z., Tenente, I. M., Clagg, R., ... Langenau, D. M. (2012). In Vivo Imaging of Tumor-Propagating Cells, Regional Tumor Heterogeneity, and Dynamic Cell Movements in Embryonal Rhabdomyosarcoma. Cancer Cell, 21(5), 680-693. https://doi.org/10.1016/j.ccr.2012.03.043

In Vivo Imaging of Tumor-Propagating Cells, Regional Tumor Heterogeneity, and Dynamic Cell Movements in Embryonal Rhabdomyosarcoma. / Ignatius, Myron S.; Chen, Eleanor; Elpek, Natalie M.; Fuller, Adam Z.; Tenente, Inês M.; Clagg, Ryan; Liu, Sali; Blackburn, Jessica S.; Linardic, Corinne M.; Rosenberg, Andrew; Nielsen, Petur G.; Mempel, Thorsten R.; Langenau, David M.

In: Cancer Cell, Vol. 21, No. 5, 25.05.2012, p. 680-693.

Research output: Contribution to journalArticle

Ignatius, MS, Chen, E, Elpek, NM, Fuller, AZ, Tenente, IM, Clagg, R, Liu, S, Blackburn, JS, Linardic, CM, Rosenberg, A, Nielsen, PG, Mempel, TR & Langenau, DM 2012, 'In Vivo Imaging of Tumor-Propagating Cells, Regional Tumor Heterogeneity, and Dynamic Cell Movements in Embryonal Rhabdomyosarcoma', Cancer Cell, vol. 21, no. 5, pp. 680-693. https://doi.org/10.1016/j.ccr.2012.03.043
Ignatius, Myron S. ; Chen, Eleanor ; Elpek, Natalie M. ; Fuller, Adam Z. ; Tenente, Inês M. ; Clagg, Ryan ; Liu, Sali ; Blackburn, Jessica S. ; Linardic, Corinne M. ; Rosenberg, Andrew ; Nielsen, Petur G. ; Mempel, Thorsten R. ; Langenau, David M. / In Vivo Imaging of Tumor-Propagating Cells, Regional Tumor Heterogeneity, and Dynamic Cell Movements in Embryonal Rhabdomyosarcoma. In: Cancer Cell. 2012 ; Vol. 21, No. 5. pp. 680-693.
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