In vivo imaging of C. elegans mechanosensory neurons demonstrates a specific role for the MEC-4 channel in the process of gentle touch sensation

Hiroshi Suzuki, Rex Kerr, Laura Bianchi, Christian Frøkjær-Jensen, Dan Slone, Jian Xue, Beate Gerstbrein, Monica Driscoll, William R. Schafer

Research output: Contribution to journalArticle

193 Citations (Scopus)

Abstract

In the nematode C. elegans, genes encoding components of a putative mechanotransducing channel complex have been identified in screens for light-touch-insensitive mutants. A long-standing question, however, is whether identified MEC proteins act directly in touch transduction or contribute indirectly by maintaining basic mechanoreceptor neuron physiology. In this study, we used the genetically encoded calcium indicator cameleon to record cellular responses of mechanosensory neurons to touch stimuli in intact, behaving nematodes. We defined a gentle touch sensory modality that adapts with a time course of approximately 500 ms and primarily senses motion rather than pressure. The DEG/ENaC channel subunit MEC-4 and channel-associated stomatin MEC-2 are specifically required for neural responses to gentle mechanical stimulation, but do not affect the basic physiology of touch neurons or their in vivo responses to harsh mechanical stimulation. These results distinguish a specific role for the MEC channel proteins in the process of gentle touch mechanosensation.

Original languageEnglish
Pages (from-to)1005-1017
Number of pages13
JournalNeuron
Volume39
Issue number6
DOIs
StatePublished - Sep 11 2003
Externally publishedYes

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Touch
Neurons
Gene Components
Mechanoreceptors
Proteins
Calcium
Pressure

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

In vivo imaging of C. elegans mechanosensory neurons demonstrates a specific role for the MEC-4 channel in the process of gentle touch sensation. / Suzuki, Hiroshi; Kerr, Rex; Bianchi, Laura; Frøkjær-Jensen, Christian; Slone, Dan; Xue, Jian; Gerstbrein, Beate; Driscoll, Monica; Schafer, William R.

In: Neuron, Vol. 39, No. 6, 11.09.2003, p. 1005-1017.

Research output: Contribution to journalArticle

Suzuki, H, Kerr, R, Bianchi, L, Frøkjær-Jensen, C, Slone, D, Xue, J, Gerstbrein, B, Driscoll, M & Schafer, WR 2003, 'In vivo imaging of C. elegans mechanosensory neurons demonstrates a specific role for the MEC-4 channel in the process of gentle touch sensation', Neuron, vol. 39, no. 6, pp. 1005-1017. https://doi.org/10.1016/j.neuron.2003.08.015
Suzuki, Hiroshi ; Kerr, Rex ; Bianchi, Laura ; Frøkjær-Jensen, Christian ; Slone, Dan ; Xue, Jian ; Gerstbrein, Beate ; Driscoll, Monica ; Schafer, William R. / In vivo imaging of C. elegans mechanosensory neurons demonstrates a specific role for the MEC-4 channel in the process of gentle touch sensation. In: Neuron. 2003 ; Vol. 39, No. 6. pp. 1005-1017.
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