In vivo evaluation of acellular human dermis for abdominal wall repair

Daniel Eberli, Sergio Rodriguez, Anthony Atala, James J. Yoo

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Limitations of synthetic biomaterials for abdominal wall repair have led investigators to seek naturally derived matrices, such as human acellular dermis, because of their excellent biocompatibility and their ability to naturally interface with host tissues with minimal tissue response. In this study, we investigated two different biomaterials derived from human dermis (FlexHD acellular dermis and FlexHD acellular dermis-thick) in a rabbit abdominal hernia repair model. One quarter of the abdominal wall was replaced with each biomaterial, and the animals were followed for up to 24 weeks. Rabbit hernias repaired with AlloDerm®, a commercially available acellular dermal matrix, and sham operated animals served as controls. Retrieved samples of these implants were assessed grossly and histologically. Collagen production measurements and tension studies were performed. FlexHD acellular dermis, FlexHD acellular dermis-thick, and AlloDerm® maintained their strength in the rabbit hernia repair model with no incidence of hernia formation or bowel adhesion. The exact size measurements at 24 weeks were 217.0 ± 20.9% for FlexHD acellular dermis, 200.8 ± 23.5% for FlexHD acellular dermisthick, and 209.7 ± 32.9% for AlloDerm®. Macroscopic and microscopic evaluation showed excellent integration and tissue formation. All biomaterials studied harbored cells that produced new collagen fibers, and a six-fold increase in these fibers was observed at 24 weeks. This study shows that acellular biomaterials derived from human dermis are suitable for abdominal hernia repair.

Original languageEnglish
Pages (from-to)1527-1538
Number of pages12
JournalJournal of Biomedical Materials Research - Part A
Volume93
Issue number4
DOIs
StatePublished - Jun 15 2010
Externally publishedYes

Fingerprint

Biomaterials
Biocompatible Materials
Repair
Tissue
Collagen
Animals
Fibers
Biocompatibility
Adhesion
FlexHD
Alloderm

Keywords

  • Acellular matrices
  • Biomaterials
  • Hernia repair
  • Human dermis

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

Cite this

In vivo evaluation of acellular human dermis for abdominal wall repair. / Eberli, Daniel; Rodriguez, Sergio; Atala, Anthony; Yoo, James J.

In: Journal of Biomedical Materials Research - Part A, Vol. 93, No. 4, 15.06.2010, p. 1527-1538.

Research output: Contribution to journalArticle

Eberli, Daniel ; Rodriguez, Sergio ; Atala, Anthony ; Yoo, James J. / In vivo evaluation of acellular human dermis for abdominal wall repair. In: Journal of Biomedical Materials Research - Part A. 2010 ; Vol. 93, No. 4. pp. 1527-1538.
@article{448bfee5950c4ff6aa4f4c853d91645e,
title = "In vivo evaluation of acellular human dermis for abdominal wall repair",
abstract = "Limitations of synthetic biomaterials for abdominal wall repair have led investigators to seek naturally derived matrices, such as human acellular dermis, because of their excellent biocompatibility and their ability to naturally interface with host tissues with minimal tissue response. In this study, we investigated two different biomaterials derived from human dermis (FlexHD acellular dermis and FlexHD acellular dermis-thick) in a rabbit abdominal hernia repair model. One quarter of the abdominal wall was replaced with each biomaterial, and the animals were followed for up to 24 weeks. Rabbit hernias repaired with AlloDerm{\circledR}, a commercially available acellular dermal matrix, and sham operated animals served as controls. Retrieved samples of these implants were assessed grossly and histologically. Collagen production measurements and tension studies were performed. FlexHD acellular dermis, FlexHD acellular dermis-thick, and AlloDerm{\circledR} maintained their strength in the rabbit hernia repair model with no incidence of hernia formation or bowel adhesion. The exact size measurements at 24 weeks were 217.0 ± 20.9{\%} for FlexHD acellular dermis, 200.8 ± 23.5{\%} for FlexHD acellular dermisthick, and 209.7 ± 32.9{\%} for AlloDerm{\circledR}. Macroscopic and microscopic evaluation showed excellent integration and tissue formation. All biomaterials studied harbored cells that produced new collagen fibers, and a six-fold increase in these fibers was observed at 24 weeks. This study shows that acellular biomaterials derived from human dermis are suitable for abdominal hernia repair.",
keywords = "Acellular matrices, Biomaterials, Hernia repair, Human dermis",
author = "Daniel Eberli and Sergio Rodriguez and Anthony Atala and Yoo, {James J.}",
year = "2010",
month = "6",
day = "15",
doi = "10.1002/jbm.a.32636",
language = "English",
volume = "93",
pages = "1527--1538",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "1549-3296",
publisher = "Heterocorporation",
number = "4",

}

TY - JOUR

T1 - In vivo evaluation of acellular human dermis for abdominal wall repair

AU - Eberli, Daniel

AU - Rodriguez, Sergio

AU - Atala, Anthony

AU - Yoo, James J.

PY - 2010/6/15

Y1 - 2010/6/15

N2 - Limitations of synthetic biomaterials for abdominal wall repair have led investigators to seek naturally derived matrices, such as human acellular dermis, because of their excellent biocompatibility and their ability to naturally interface with host tissues with minimal tissue response. In this study, we investigated two different biomaterials derived from human dermis (FlexHD acellular dermis and FlexHD acellular dermis-thick) in a rabbit abdominal hernia repair model. One quarter of the abdominal wall was replaced with each biomaterial, and the animals were followed for up to 24 weeks. Rabbit hernias repaired with AlloDerm®, a commercially available acellular dermal matrix, and sham operated animals served as controls. Retrieved samples of these implants were assessed grossly and histologically. Collagen production measurements and tension studies were performed. FlexHD acellular dermis, FlexHD acellular dermis-thick, and AlloDerm® maintained their strength in the rabbit hernia repair model with no incidence of hernia formation or bowel adhesion. The exact size measurements at 24 weeks were 217.0 ± 20.9% for FlexHD acellular dermis, 200.8 ± 23.5% for FlexHD acellular dermisthick, and 209.7 ± 32.9% for AlloDerm®. Macroscopic and microscopic evaluation showed excellent integration and tissue formation. All biomaterials studied harbored cells that produced new collagen fibers, and a six-fold increase in these fibers was observed at 24 weeks. This study shows that acellular biomaterials derived from human dermis are suitable for abdominal hernia repair.

AB - Limitations of synthetic biomaterials for abdominal wall repair have led investigators to seek naturally derived matrices, such as human acellular dermis, because of their excellent biocompatibility and their ability to naturally interface with host tissues with minimal tissue response. In this study, we investigated two different biomaterials derived from human dermis (FlexHD acellular dermis and FlexHD acellular dermis-thick) in a rabbit abdominal hernia repair model. One quarter of the abdominal wall was replaced with each biomaterial, and the animals were followed for up to 24 weeks. Rabbit hernias repaired with AlloDerm®, a commercially available acellular dermal matrix, and sham operated animals served as controls. Retrieved samples of these implants were assessed grossly and histologically. Collagen production measurements and tension studies were performed. FlexHD acellular dermis, FlexHD acellular dermis-thick, and AlloDerm® maintained their strength in the rabbit hernia repair model with no incidence of hernia formation or bowel adhesion. The exact size measurements at 24 weeks were 217.0 ± 20.9% for FlexHD acellular dermis, 200.8 ± 23.5% for FlexHD acellular dermisthick, and 209.7 ± 32.9% for AlloDerm®. Macroscopic and microscopic evaluation showed excellent integration and tissue formation. All biomaterials studied harbored cells that produced new collagen fibers, and a six-fold increase in these fibers was observed at 24 weeks. This study shows that acellular biomaterials derived from human dermis are suitable for abdominal hernia repair.

KW - Acellular matrices

KW - Biomaterials

KW - Hernia repair

KW - Human dermis

UR - http://www.scopus.com/inward/record.url?scp=77951569865&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951569865&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.32636

DO - 10.1002/jbm.a.32636

M3 - Article

C2 - 20014294

AN - SCOPUS:77951569865

VL - 93

SP - 1527

EP - 1538

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 1549-3296

IS - 4

ER -