In vivo and in organello assessment of OXPHOS activities

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Abstract

Mitochondrial OXPHOS defects are responsible for a large group of human diseases and have been associated with degenerative disorders and aging. The accurate in vivo and in organello biochemical assessment of the OXPHOS system is necessary for the diagnosis and investigation of such conditions. Here I describe a set of accurate polarographic and spectrophotometric assays that use relatively small amounts of biological material (cells or isolated mitochondria) and discuss the biochemical parameters appropriate for discriminating partial OXPHOS defects.

Original languageEnglish (US)
Pages (from-to)307-316
Number of pages10
JournalMethods
Volume26
Issue number4
DOIs
StatePublished - Sep 12 2002
Externally publishedYes

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Keywords

  • Cell membrane permeabilization
  • Complexes I-V
  • Mitochondria
  • Mitochondrial disease
  • Mitochondrial membrane disruption
  • Mitochondrial respiratory chain
  • OXPHOS
  • Oxygen consumption
  • Polarography

ASJC Scopus subject areas

  • Molecular Biology

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