In vitro uptake of amphiphilic, hydrogel nanoparticles by J774A.1 cells

Dimitris Missirlis, Jeffrey A. Hubbell

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


We here report improved synthesis and in vitro interactions of amphiphilic hydrogel nanoparticles with the macrophage cell line J774A.1. Nanoparticles comprising dispersed hydrophobic nanodomains of poly(propylene glycol) within a continuous phase of hydrophilic poly (ethylene glycol) (PEG) were prepared via inverse emulsion crosslinking polymerization, using acrylated PEG and Pluronic® F127 as macromonomer blocks. Functionality and fluorescent labeling were achieved through incorporation of reactive comonomers and a posteriori reaction with fluorescein, respectively. When introduced to a static cell culture of adhered J774A.1 macrophages, the cells internalized these hydrogel nanoparticles in a dose- and time-dependent manner through clathrin-mediated and other pathways. Amphiphilic nanoparticle uptake was however dramatically lower than that of a model system (Fluospheres®) and similar to PEG-coated colloids reported in the literature, which are considered "stealth." Our findings support the potential of the nanoparticles presented here as long-circulating drug carriers.

Original languageEnglish (US)
Pages (from-to)1557-1565
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Issue number4
StatePublished - Jun 15 2010


  • Copolymer
  • Hydrophilicity
  • Internalization
  • Inverse emulsion
  • Stealth carrier

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys


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