In Vitro Synthesis of Normal Bone (Type I) Collagen by Bones of Paget's Disease Patients

Herman S. Cheung, Frederick R. Singer, Barbara Mills, Marcel E. Nimni

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Bone samples were obtained from five Paget's disease patients and one normal individual at surgery. We characterized the collagen content of bone samples chemically, using sodium dodecyl sulfate gel electrophoresis and cyanogen bromide peptide analysis, and also determined the type of collagen produced in vitro by these samples. Both Pagetic and normal bones make normal bone collagen with the α1 to α2 chains ratio equal to 2:1. However, Pagetic bones synthesized three to four times more protein and collagen per milligram of tissue as compared to normal bones.

Original languageEnglish (US)
Pages (from-to)547-552
Number of pages6
JournalProceedings of the Society for Experimental Biology and Medicine
Volume163
Issue number4
DOIs
StatePublished - Apr 1980
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'In Vitro Synthesis of Normal Bone (Type I) Collagen by Bones of Paget's Disease Patients'. Together they form a unique fingerprint.

  • Cite this