In vitro Studies with Thyrotropin Releasing Factor

Andrew V. Schally, Tommie W. Redding

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Porcine TRF stimulates the release of TSH from rat anterior pituitaries in vitro at doses as small as 0.01 nanog. By increasing the doses of TRF, greater amounts of TSH are released into the incubation media. The pituitary response to TRF is inhibited by small amounts of T3 and T4. Act D does not abolish the response to TRF, indicating that de novo synthesis of TSH is not required for TRF to exert its effect, a- and β-MSH do not stimulate the release of TSH in vitro and did not reverse the inhibitory effect of T4 on TSH release in vitro. Preincubation with Act D reverses the inhibition of TSH release induced by T3 and T4. This may indicate that Act D interferes with the formation of inhibitory substances, induced by T3 and T4, which suppress the release of TSH after TRF.

Original languageEnglish (US)
Pages (from-to)320-325
Number of pages6
JournalProceedings of the Society for Experimental Biology and Medicine
Issue number1
StatePublished - Oct 1967
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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