In vitro properties of RpoS (σs) mutants of Escherichia coli with postulated N-terminal subregion 1.1 or C-terminal region 4 deleted

J. Gowrishankar, Kaneyoshi Yamamoto, Subbayan Pochi, Akira Ishihama

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Derivatives of the stationary-phase sigma factor σs of Escherichia coli lacking either of two conserved domains, the postulated N-terminal subregion 1.1 or the C-terminal region 4, were shown to be competent in vitro for transcription initiation from several σs-dependent promoters on supercoiled DNA templates. Unlike wild-type σs, however, the deletion derivatives were inactive on relaxed templates. The anomalous slow electrophoretic mobility of σs on denaturing gels was corrected by deletion of subregion 1.1, suggesting that this domain in σs may be structurally and functionally analogous to subregion 1.1 of σ70, substitutions in which have previously been shown to rectify the anomalous electrophoretic migration of σ70 (V. Gopal and D. Chatterji, Eur. J. Biochem. 244:614-618, 1997).

Original languageEnglish
Pages (from-to)2673-2679
Number of pages7
JournalJournal of Bacteriology
Volume185
Issue number8
DOIs
StatePublished - Apr 1 2003

Fingerprint

Superhelical DNA
Sigma Factor
Gels
Escherichia coli
In Vitro Techniques

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

In vitro properties of RpoS (σs) mutants of Escherichia coli with postulated N-terminal subregion 1.1 or C-terminal region 4 deleted. / Gowrishankar, J.; Yamamoto, Kaneyoshi; Pochi, Subbayan; Ishihama, Akira.

In: Journal of Bacteriology, Vol. 185, No. 8, 01.04.2003, p. 2673-2679.

Research output: Contribution to journalArticle

Gowrishankar, J. ; Yamamoto, Kaneyoshi ; Pochi, Subbayan ; Ishihama, Akira. / In vitro properties of RpoS (σs) mutants of Escherichia coli with postulated N-terminal subregion 1.1 or C-terminal region 4 deleted. In: Journal of Bacteriology. 2003 ; Vol. 185, No. 8. pp. 2673-2679.
@article{8a6ffe2715c3419da880a558a0b663bd,
title = "In vitro properties of RpoS (σs) mutants of Escherichia coli with postulated N-terminal subregion 1.1 or C-terminal region 4 deleted",
abstract = "Derivatives of the stationary-phase sigma factor σs of Escherichia coli lacking either of two conserved domains, the postulated N-terminal subregion 1.1 or the C-terminal region 4, were shown to be competent in vitro for transcription initiation from several σs-dependent promoters on supercoiled DNA templates. Unlike wild-type σs, however, the deletion derivatives were inactive on relaxed templates. The anomalous slow electrophoretic mobility of σs on denaturing gels was corrected by deletion of subregion 1.1, suggesting that this domain in σs may be structurally and functionally analogous to subregion 1.1 of σ70, substitutions in which have previously been shown to rectify the anomalous electrophoretic migration of σ70 (V. Gopal and D. Chatterji, Eur. J. Biochem. 244:614-618, 1997).",
author = "J. Gowrishankar and Kaneyoshi Yamamoto and Subbayan Pochi and Akira Ishihama",
year = "2003",
month = "4",
day = "1",
doi = "10.1128/JB.185.8.2673-2679.2003",
language = "English",
volume = "185",
pages = "2673--2679",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "8",

}

TY - JOUR

T1 - In vitro properties of RpoS (σs) mutants of Escherichia coli with postulated N-terminal subregion 1.1 or C-terminal region 4 deleted

AU - Gowrishankar, J.

AU - Yamamoto, Kaneyoshi

AU - Pochi, Subbayan

AU - Ishihama, Akira

PY - 2003/4/1

Y1 - 2003/4/1

N2 - Derivatives of the stationary-phase sigma factor σs of Escherichia coli lacking either of two conserved domains, the postulated N-terminal subregion 1.1 or the C-terminal region 4, were shown to be competent in vitro for transcription initiation from several σs-dependent promoters on supercoiled DNA templates. Unlike wild-type σs, however, the deletion derivatives were inactive on relaxed templates. The anomalous slow electrophoretic mobility of σs on denaturing gels was corrected by deletion of subregion 1.1, suggesting that this domain in σs may be structurally and functionally analogous to subregion 1.1 of σ70, substitutions in which have previously been shown to rectify the anomalous electrophoretic migration of σ70 (V. Gopal and D. Chatterji, Eur. J. Biochem. 244:614-618, 1997).

AB - Derivatives of the stationary-phase sigma factor σs of Escherichia coli lacking either of two conserved domains, the postulated N-terminal subregion 1.1 or the C-terminal region 4, were shown to be competent in vitro for transcription initiation from several σs-dependent promoters on supercoiled DNA templates. Unlike wild-type σs, however, the deletion derivatives were inactive on relaxed templates. The anomalous slow electrophoretic mobility of σs on denaturing gels was corrected by deletion of subregion 1.1, suggesting that this domain in σs may be structurally and functionally analogous to subregion 1.1 of σ70, substitutions in which have previously been shown to rectify the anomalous electrophoretic migration of σ70 (V. Gopal and D. Chatterji, Eur. J. Biochem. 244:614-618, 1997).

UR - http://www.scopus.com/inward/record.url?scp=0037389328&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037389328&partnerID=8YFLogxK

U2 - 10.1128/JB.185.8.2673-2679.2003

DO - 10.1128/JB.185.8.2673-2679.2003

M3 - Article

C2 - 12670993

AN - SCOPUS:0037389328

VL - 185

SP - 2673

EP - 2679

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 8

ER -