In vitro ototoxicity of aminoglycosides and platin derivatives. A semi- automatic assay for sensory hair cell damage in explanted rat organ of corti

B. Malgrange; P. P. Lefebvre; T. R. Van De Water; C. Bonnet; F. Monville; J. M. Rigo; H. Staecker; G. Moonen

doi:10.1016/S0887-2333(98)00048-4

In vitro ototoxicity of aminoglycosides and platin derivatives. A semi- automatic assay for sensory hair cell damage in explanted rat organ of corti

B. Malgrange, P. P. Lefebvre, T. R. Van De Water, C. Bonnet, F. Monville, J. M. Rigo, H. Staecker, G. Moonen

Otolaryngology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The ototoxic damage that drugs such as neomycin, kanamycin, colistin, cisplatin, transplatin and carboplatin cause on outer and inner hair cells in postnatal day 3 rat cochlear explants was investigated. Phalloidin- fluorescein conjugate-stained stereocilia bundles of sensory hair cells were quantified by video image analysis as a measurement of ototoxic effect. The video image quantification system established dose-response curves for ototoxic drugs (e.g. calculation of an IC₅₀) and allowed comparisons between several ototoxins from the same family. This methodology provided the means to assess the efficacy of otoprotectant agents in preventing ototoxicity. Poly-l-aspartate (10^-5 M) and poly-l-glutamate (10^-5 M) protected auditory hair cells from neomycin (10^-3 M) toxicity while reduced glutathione (10^-3 M) provided protection against cisplatin (10^-4 M)- induced hair cell damage.

Original language	English (US)
Pages (from-to)	705-714
Number of pages	10
Journal	Toxicology in Vitro
Volume	12
Issue number	6
DOIs	https://doi.org/10.1016/S0887-2333(98)00048-4
State	Published - Dec 1998

Keywords

Aminoglycosides
Cisplatin
Cochlea
Otoprotection
Ototoxicity

ASJC Scopus subject areas

Toxicology

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10.1016/S0887-2333(98)00048-4

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In vitro ototoxicity of aminoglycosides and platin derivatives. A semi- automatic assay for sensory hair cell damage in explanted rat organ of corti. / Malgrange, B.; Lefebvre, P. P.; Van De Water, T. R.; Bonnet, C.; Monville, F.; Rigo, J. M.; Staecker, H.; Moonen, G.

In: Toxicology in Vitro, Vol. 12, No. 6, 12.1998, p. 705-714.

Research output: Contribution to journal › Article › peer-review

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title = "In vitro ototoxicity of aminoglycosides and platin derivatives. A semi- automatic assay for sensory hair cell damage in explanted rat organ of corti",

abstract = "The ototoxic damage that drugs such as neomycin, kanamycin, colistin, cisplatin, transplatin and carboplatin cause on outer and inner hair cells in postnatal day 3 rat cochlear explants was investigated. Phalloidin- fluorescein conjugate-stained stereocilia bundles of sensory hair cells were quantified by video image analysis as a measurement of ototoxic effect. The video image quantification system established dose-response curves for ototoxic drugs (e.g. calculation of an IC50) and allowed comparisons between several ototoxins from the same family. This methodology provided the means to assess the efficacy of otoprotectant agents in preventing ototoxicity. Poly-l-aspartate (10-5 M) and poly-l-glutamate (10-5 M) protected auditory hair cells from neomycin (10-3 M) toxicity while reduced glutathione (10-3 M) provided protection against cisplatin (10-4 M)- induced hair cell damage.",

keywords = "Aminoglycosides, Cisplatin, Cochlea, Otoprotection, Ototoxicity",

author = "B. Malgrange and Lefebvre, {P. P.} and {Van De Water}, {T. R.} and C. Bonnet and F. Monville and Rigo, {J. M.} and H. Staecker and G. Moonen",

note = "Funding Information: This work was supported by grants from the National Fund for Scientific Research of Belgium to BM, GM and PPL, the Concerted Action of the Government of the French Community of Belgium to BM and GM and the Queen Elisabeth Medical Foundation of Belgium to GM, National Institute for Deafness and other Communication Disorders (DC00088) and the Shulsky Fund for Hearing Research of the Montefiore Medical Center to TRV. We thank P. Ernst for her technical assistance.",

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AU - Lefebvre, P. P.

AU - Van De Water, T. R.

AU - Bonnet, C.

AU - Monville, F.

AU - Rigo, J. M.

AU - Staecker, H.

AU - Moonen, G.

N1 - Funding Information: This work was supported by grants from the National Fund for Scientific Research of Belgium to BM, GM and PPL, the Concerted Action of the Government of the French Community of Belgium to BM and GM and the Queen Elisabeth Medical Foundation of Belgium to GM, National Institute for Deafness and other Communication Disorders (DC00088) and the Shulsky Fund for Hearing Research of the Montefiore Medical Center to TRV. We thank P. Ernst for her technical assistance.

PY - 1998/12

Y1 - 1998/12

N2 - The ototoxic damage that drugs such as neomycin, kanamycin, colistin, cisplatin, transplatin and carboplatin cause on outer and inner hair cells in postnatal day 3 rat cochlear explants was investigated. Phalloidin- fluorescein conjugate-stained stereocilia bundles of sensory hair cells were quantified by video image analysis as a measurement of ototoxic effect. The video image quantification system established dose-response curves for ototoxic drugs (e.g. calculation of an IC50) and allowed comparisons between several ototoxins from the same family. This methodology provided the means to assess the efficacy of otoprotectant agents in preventing ototoxicity. Poly-l-aspartate (10-5 M) and poly-l-glutamate (10-5 M) protected auditory hair cells from neomycin (10-3 M) toxicity while reduced glutathione (10-3 M) provided protection against cisplatin (10-4 M)- induced hair cell damage.

AB - The ototoxic damage that drugs such as neomycin, kanamycin, colistin, cisplatin, transplatin and carboplatin cause on outer and inner hair cells in postnatal day 3 rat cochlear explants was investigated. Phalloidin- fluorescein conjugate-stained stereocilia bundles of sensory hair cells were quantified by video image analysis as a measurement of ototoxic effect. The video image quantification system established dose-response curves for ototoxic drugs (e.g. calculation of an IC50) and allowed comparisons between several ototoxins from the same family. This methodology provided the means to assess the efficacy of otoprotectant agents in preventing ototoxicity. Poly-l-aspartate (10-5 M) and poly-l-glutamate (10-5 M) protected auditory hair cells from neomycin (10-3 M) toxicity while reduced glutathione (10-3 M) provided protection against cisplatin (10-4 M)- induced hair cell damage.

KW - Aminoglycosides

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In vitro ototoxicity of aminoglycosides and platin derivatives. A semi- automatic assay for sensory hair cell damage in explanted rat organ of corti

Abstract

Keywords

ASJC Scopus subject areas

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