In vitro ototoxicity of aminoglycosides and platin derivatives. A semi- automatic assay for sensory hair cell damage in explanted rat organ of corti

B. Malgrange, P. P. Lefebvre, Thomas R Van De Water, C. Bonnet, F. Monville, J. M. Rigo, H. Staecker, G. Moonen

Research output: Contribution to journal › Article

3 Citations (Scopus)

Abstract

The ototoxic damage that drugs such as neomycin, kanamycin, colistin, cisplatin, transplatin and carboplatin cause on outer and inner hair cells in postnatal day 3 rat cochlear explants was investigated. Phalloidin- fluorescein conjugate-stained stereocilia bundles of sensory hair cells were quantified by video image analysis as a measurement of ototoxic effect. The video image quantification system established dose-response curves for ototoxic drugs (e.g. calculation of an IC50) and allowed comparisons between several ototoxins from the same family. This methodology provided the means to assess the efficacy of otoprotectant agents in preventing ototoxicity. Poly-l-aspartate (10-5 M) and poly-l-glutamate (10-5 M) protected auditory hair cells from neomycin (10-3 M) toxicity while reduced glutathione (10-3 M) provided protection against cisplatin (10-4 M)- induced hair cell damage.

Original languageEnglish
Pages (from-to)705-714
Number of pages10
JournalToxicology in Vitro
Volume12
Issue number6
DOIs
StatePublished - Dec 1 1998
Externally publishedYes

Fingerprint

Organ of Corti
Neomycin
Aminoglycosides
Outer Auditory Hair Cells
Inner Auditory Hair Cells
Cisplatin
Rats
Assays
Auditory Hair Cells
Cells
Stereocilia
Derivatives
Colistin
Phalloidine
Kanamycin
Carboplatin
Cochlea
Fluorescein
Aspartic Acid
Pharmaceutical Preparations

Keywords

  • Aminoglycosides
  • Cisplatin
  • Cochlea
  • Otoprotection
  • Ototoxicity

ASJC Scopus subject areas

  • Toxicology

Cite this

In vitro ototoxicity of aminoglycosides and platin derivatives. A semi- automatic assay for sensory hair cell damage in explanted rat organ of corti. / Malgrange, B.; Lefebvre, P. P.; Van De Water, Thomas R; Bonnet, C.; Monville, F.; Rigo, J. M.; Staecker, H.; Moonen, G.

In: Toxicology in Vitro, Vol. 12, No. 6, 01.12.1998, p. 705-714.

Research output: Contribution to journal › Article

Malgrange, B, Lefebvre, PP, Van De Water, TR, Bonnet, C, Monville, F, Rigo, JM, Staecker, H & Moonen, G 1998, 'In vitro ototoxicity of aminoglycosides and platin derivatives. A semi- automatic assay for sensory hair cell damage in explanted rat organ of corti', Toxicology in Vitro, vol. 12, no. 6, pp. 705-714. https://doi.org/10.1016/S0887-2333(98)00048-4
Malgrange B, Lefebvre PP, Van De Water TR, Bonnet C, Monville F, Rigo JM et al. In vitro ototoxicity of aminoglycosides and platin derivatives. A semi- automatic assay for sensory hair cell damage in explanted rat organ of corti. Toxicology in Vitro. 1998 Dec 1;12(6):705-714. https://doi.org/10.1016/S0887-2333(98)00048-4
Malgrange, B. ; Lefebvre, P. P. ; Van De Water, Thomas R ; Bonnet, C. ; Monville, F. ; Rigo, J. M. ; Staecker, H. ; Moonen, G. / In vitro ototoxicity of aminoglycosides and platin derivatives. A semi- automatic assay for sensory hair cell damage in explanted rat organ of corti. In: Toxicology in Vitro. 1998 ; Vol. 12, No. 6. pp. 705-714.
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