In vitro mutagenesis of the human T-cell leukemia virus types I and II tax genes

A. J. Cann, Joseph D Rosenblatt, W. Wachsman, I. S Y Chen

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The tax gene of the human T-cell leukemia virus types I and II (HTLV-I and HTLV-II) is essential for viral replication and acts by increasing the level of RNA transcription. The tax genes of HTLV-I and HTLV-II encode proteins of 40 and 37 kilodaltons, respectively. By in vitro mutagenesis of the tax gene, we have investigated those regions of the protein which are essential for its function. Mutation of either the amino- or carboxy-terminal domain of the protein resulted in loss of trans-activation ability. In addition, specificity of its activity with regard to trans-activation of either the HTLV-I or HTLV-II long terminal repeats was conferred by the first 59 amino acids.

Original languageEnglish
Pages (from-to)1474-1479
Number of pages6
JournalJournal of Virology
Volume63
Issue number3
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Primate T-lymphotropic virus 1
pX Genes
Human T-lymphotropic virus 2
Human T-lymphotropic virus 1
taxes
mutagenesis
Mutagenesis
transcriptional activation
terminal repeat sequences
protein depletion
genes
virus replication
Terminal Repeat Sequences
proteins
transcription (genetics)
RNA
mutation
Proteins
amino acids
Amino Acids

ASJC Scopus subject areas

  • Immunology

Cite this

In vitro mutagenesis of the human T-cell leukemia virus types I and II tax genes. / Cann, A. J.; Rosenblatt, Joseph D; Wachsman, W.; Chen, I. S Y.

In: Journal of Virology, Vol. 63, No. 3, 01.01.1989, p. 1474-1479.

Research output: Contribution to journalArticle

Cann, A. J. ; Rosenblatt, Joseph D ; Wachsman, W. ; Chen, I. S Y. / In vitro mutagenesis of the human T-cell leukemia virus types I and II tax genes. In: Journal of Virology. 1989 ; Vol. 63, No. 3. pp. 1474-1479.
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