Abstract
A variety of in vitro models have been developed to understand the mechanisms underlying the regenerative failure of central nervous system (CNS) axons, and to guide pre-clinical development of regeneration-promoting therapeutics. These range from single-cell based assays that typically focus on molecular mechanisms to organotypic assays that aim to recapitulate in vivo behavior. By utilizing a combination of models, researchers can balance the speed, convenience, and mechanistic resolution of simpler models with the biological relevance of more complex models. This review will discuss a number of models that have been used to build our understanding of the molecular mechanisms of CNS axon regeneration.
Original language | English (US) |
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Pages (from-to) | 423-434 |
Number of pages | 12 |
Journal | Experimental neurology |
Volume | 287 |
DOIs | |
State | Published - Jan 1 2017 |
Keywords
- Axon regeneration
- Cell based assays
- High content screening
- In vitro models
- Neurite outgrowth
- Organotypic assays
ASJC Scopus subject areas
- Neurology
- Developmental Neuroscience