In vitro models of axon regeneration

Hassan Al-Ali; Samuel R. Beckerman; John L. Bixby; Vance P. Lemmon

doi:10.1016/j.expneurol.2016.01.020

In vitro models of axon regeneration

Hassan Al-Ali, Samuel R. Beckerman, John L. Bixby, Vance P. Lemmon

Research output: Contribution to journal › Review article › peer-review

21 Scopus citations

Abstract

A variety of in vitro models have been developed to understand the mechanisms underlying the regenerative failure of central nervous system (CNS) axons, and to guide pre-clinical development of regeneration-promoting therapeutics. These range from single-cell based assays that typically focus on molecular mechanisms to organotypic assays that aim to recapitulate in vivo behavior. By utilizing a combination of models, researchers can balance the speed, convenience, and mechanistic resolution of simpler models with the biological relevance of more complex models. This review will discuss a number of models that have been used to build our understanding of the molecular mechanisms of CNS axon regeneration.

Original language	English (US)
Pages (from-to)	423-434
Number of pages	12
Journal	Experimental neurology
Volume	287
DOIs	https://doi.org/10.1016/j.expneurol.2016.01.020
State	Published - Jan 1 2017

Keywords

Axon regeneration
Cell based assays
High content screening
In vitro models
Neurite outgrowth
Organotypic assays

ASJC Scopus subject areas

Neurology
Developmental Neuroscience

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title = "In vitro models of axon regeneration",

abstract = "A variety of in vitro models have been developed to understand the mechanisms underlying the regenerative failure of central nervous system (CNS) axons, and to guide pre-clinical development of regeneration-promoting therapeutics. These range from single-cell based assays that typically focus on molecular mechanisms to organotypic assays that aim to recapitulate in vivo behavior. By utilizing a combination of models, researchers can balance the speed, convenience, and mechanistic resolution of simpler models with the biological relevance of more complex models. This review will discuss a number of models that have been used to build our understanding of the molecular mechanisms of CNS axon regeneration.",

keywords = "Axon regeneration, Cell based assays, High content screening, In vitro models, Neurite outgrowth, Organotypic assays",

author = "Hassan Al-Ali and Beckerman, {Samuel R.} and Bixby, {John L.} and Lemmon, {Vance P.}",

note = "Funding Information: This work was supported by the National Institute of Child Health and Human Development [grant number HD057632] and the National Institute of Neurological Disorders and Stroke [grant number NS080145], as well as the Miami Project to Cure Paralysis and the Walter G. Ross Foundation.",

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AU - Al-Ali, Hassan

AU - Beckerman, Samuel R.

AU - Bixby, John L.

AU - Lemmon, Vance P.

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N2 - A variety of in vitro models have been developed to understand the mechanisms underlying the regenerative failure of central nervous system (CNS) axons, and to guide pre-clinical development of regeneration-promoting therapeutics. These range from single-cell based assays that typically focus on molecular mechanisms to organotypic assays that aim to recapitulate in vivo behavior. By utilizing a combination of models, researchers can balance the speed, convenience, and mechanistic resolution of simpler models with the biological relevance of more complex models. This review will discuss a number of models that have been used to build our understanding of the molecular mechanisms of CNS axon regeneration.

AB - A variety of in vitro models have been developed to understand the mechanisms underlying the regenerative failure of central nervous system (CNS) axons, and to guide pre-clinical development of regeneration-promoting therapeutics. These range from single-cell based assays that typically focus on molecular mechanisms to organotypic assays that aim to recapitulate in vivo behavior. By utilizing a combination of models, researchers can balance the speed, convenience, and mechanistic resolution of simpler models with the biological relevance of more complex models. This review will discuss a number of models that have been used to build our understanding of the molecular mechanisms of CNS axon regeneration.

KW - Axon regeneration

KW - Cell based assays

KW - High content screening

KW - In vitro models

KW - Neurite outgrowth

KW - Organotypic assays

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EP - 434

JO - Experimental Neurology

JF - Experimental Neurology

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In vitro models of axon regeneration

Abstract

Keywords

ASJC Scopus subject areas

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