In vitro microdistraction of preosteoblasts: Distraction promotes proliferation and oscillation promotes differentiation

Joubin S. Gabbay, Patricia A. Zuk, Amir Tahernia, Morad Askari, Catherine M. O'Hara, Tharun Karthikeyan, Kodi Azari, Jeffery O. Hollinger, James P. Bradley

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Osteoblast biology is influenced in vivo by a 3-dimensional (3D) extracellular matrix that mediates their adhesion and interaction and by a constant state of compressive and tensile forces. To study the role of mechanical stress on osteoblasts in vitro, these parameters must be addressed. Therefore, this study describes the use of a novel, in vitro system that subjects cells to distractive and compressive forces in a 3D environment. This system, termed a microdistractor system, was used to apply linear forces to 3D collagen type I gels containing preosteoblasts. Gels were induced for up to 16 days in osteogenic medium and subjected to either constant linear distraction (distraction gels) or to repeating cycles of distraction and compression (oscillation gels). The effect of these stresses was evaluated over time by measuring proliferation rates, protein synthesis (i.e., cellular activity), and osteogenic differentiation levels. While linear forces in general appeared to increase protein synthesis, force-specific effects on proliferation and differentiation were observed. Specifically, distraction forces appeared to enhance MC3T3 proliferation while distraction/compressive forces appeared to accelerate their osteogenic differentiation program. Therefore, these results suggest that the microdistraction system may be an appropriate in vitro system for the study of mechanobiology in osteoblast phenotype.

Original languageEnglish (US)
Pages (from-to)3055-3065
Number of pages11
JournalTissue Engineering
Issue number11
StatePublished - Nov 1 2006

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Cell Biology


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