In vitro laser welding of amniotic membranes

Gustavo A. Mendoza, Edgar Acuña, Mary Allen, Jorge Arroyo, Rubén A. Quintero

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective: To test in vitro the feasibility of welding amniotic membranes using Nd:YAG laser energy. Study design: Fresh fetal membranes from term pregnancies were washed and cut into 1 cm2 pieces. Pooled cryoprecipitate (CPT), 50% albumin (Alb), or polytetrafluoroethilene (e- PTFE) were used as solder medium. The optimal settings of the laser were determined. Results were assessed quantitatively and semi-quantitatively using Pearson Chi-square analysis. Results: Laser welding of amniotic membranes was successful in 82.6% of experiments with e-PTFE and in 10.7% of experiments with CPT (P < 0.001). The strength of the welding was also significantly better with e-PTFE (P < 0.001). Optimal results were obtained using 1-7 Watts and 0.1-1 seconds. Laser welding was unsuccessful in 100% of experiments with Alb. Conclusions: Laser welding of fetal membranes can be accomplished with e-PTFE and to a lesser degree with the CPT using Nd:YAG energy under low wattage-high exposure settings. Further studies are underway to test other grafting or soldering materials.

Original languageEnglish
Pages (from-to)315-318
Number of pages4
JournalLasers in Surgery and Medicine
Volume24
Issue number5
DOIs
StatePublished - Jul 17 1999

Fingerprint

Welding
Amnion
Polytetrafluoroethylene
Lasers
Extraembryonic Membranes
Albumins
Solid-State Lasers
In Vitro Techniques
Pregnancy

Keywords

  • Amnion
  • Nd:YAG
  • Premature rupture of membranes
  • Tissue welding

ASJC Scopus subject areas

  • Surgery

Cite this

In vitro laser welding of amniotic membranes. / Mendoza, Gustavo A.; Acuña, Edgar; Allen, Mary; Arroyo, Jorge; Quintero, Rubén A.

In: Lasers in Surgery and Medicine, Vol. 24, No. 5, 17.07.1999, p. 315-318.

Research output: Contribution to journalArticle

Mendoza, GA, Acuña, E, Allen, M, Arroyo, J & Quintero, RA 1999, 'In vitro laser welding of amniotic membranes', Lasers in Surgery and Medicine, vol. 24, no. 5, pp. 315-318. https://doi.org/10.1002/(SICI)1096-9101(1999)24:5<315::AID-LSM1>3.0.CO;2-1
Mendoza, Gustavo A. ; Acuña, Edgar ; Allen, Mary ; Arroyo, Jorge ; Quintero, Rubén A. / In vitro laser welding of amniotic membranes. In: Lasers in Surgery and Medicine. 1999 ; Vol. 24, No. 5. pp. 315-318.
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