In vitro laser welding of amniotic membranes

Gustavo A. Mendoza, Edgar Acuña, Mary Allen, Jorge Arroyo, Rubén A. Quintero

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Objective: To test in vitro the feasibility of welding amniotic membranes using Nd:YAG laser energy. Study design: Fresh fetal membranes from term pregnancies were washed and cut into 1 cm2 pieces. Pooled cryoprecipitate (CPT), 50% albumin (Alb), or polytetrafluoroethilene (e- PTFE) were used as solder medium. The optimal settings of the laser were determined. Results were assessed quantitatively and semi-quantitatively using Pearson Chi-square analysis. Results: Laser welding of amniotic membranes was successful in 82.6% of experiments with e-PTFE and in 10.7% of experiments with CPT (P < 0.001). The strength of the welding was also significantly better with e-PTFE (P < 0.001). Optimal results were obtained using 1-7 Watts and 0.1-1 seconds. Laser welding was unsuccessful in 100% of experiments with Alb. Conclusions: Laser welding of fetal membranes can be accomplished with e-PTFE and to a lesser degree with the CPT using Nd:YAG energy under low wattage-high exposure settings. Further studies are underway to test other grafting or soldering materials.

Original languageEnglish (US)
Pages (from-to)315-318
Number of pages4
JournalLasers in Surgery and Medicine
Volume24
Issue number5
DOIs
StatePublished - Jan 1 1999

Keywords

  • Amnion
  • Nd:YAG
  • Premature rupture of membranes
  • Tissue welding

ASJC Scopus subject areas

  • Surgery

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