In vitro inhibition of ocular cell proliferation with Ara-C: Blockage of the antiproliferative effect with 2'-deoxycytidine

A. S. Hajek, R. K. Parrish, K. S. Mallick, M. Gressel

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Cytosine arabinoside (ara-C), a cell cycle-phase specific antimetabolite, was tested in a tissue culture model of human scleral fibroblasts (HSCF), and human retinal pigmented epithelium cells (HRPE), to investigate possible applications in the modulation of wound healing after glaucoma filtering surgery and in the postoperative management of proliferative vitreoretinopathy. Cell proliferation after a 72 hr incubation in the presence of ara-C was inhibited to 50% of the control growth rate (ID50) with calculated doses of 60 ng/ml in HSCF and 4 and 5 ng/ml in HRPE cultures. 2'-deoxycytidine, a competitive inhibitor of ara-C action, completely eliminated the cell growth retardation of both cell types produced by ara-C after 72 hr incubation.

Original languageEnglish (US)
Pages (from-to)1010-1012
Number of pages3
JournalInvestigative Ophthalmology and Visual Science
Volume27
Issue number6
StatePublished - 1986

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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