In Vitro Immune Responses to Viral and Tumor Antigens in Murine Breast Cancer

M. Michael Sigel, Diana M. Lopez, Gabriel Ortiz-Muniz

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Inhibition of migration of peritoneal exudate cells proved to be a useful measurement of cell mediated immunity which correlated in several respects with blastogenic transformation reactions. Lectins (phytohemagglutinin and concanavalin A) inhibited the migration of peritoneal exudate cells from normal and tumor bearing mice, whereas tumor antigen caused inhibition of migration of cells from tumor bearing animals only. The disparity in immunogenic capacity previously observed with lymphocyte transformation studies was also manifested in migration inhibition, i.e., D1 DMBA 3 tumor being immunogenic and D1 DMBA 2 being nonimmunogenic. Using the migration inhibition and blastogenic transformation reactions, responses were obtained to mammary tumor virus (MTV) antigen(s) in cells from BALB/cCrgl mice, which are free of MTV. In contrast, cells from MTV positive BALB/cfC3H mice failed to respond to this antigen(s) in both reactions, suggesting a form of tolerance. However, the reactions became positive after implantation with MTV containing spontaneous mammary tumors. Two possible explanations of the origin of reactive lymphocytes, horizontal transmission, or activation of a gene coding for an MTV antigen(s), are discussed.

Original languageEnglish (US)
Pages (from-to)748-752
Number of pages5
JournalCancer Research
Volume36
StatePublished - Feb 1976

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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