In vitro identification of Rns-regulated genes

George P. Munson, Lisa G. Holcomb, Heather L. Alexander, June R. Scott

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


To identify Rns-regulated genes, a maltose binding protein (MBP)-Rns fusion protein was used to isolate DNA fragments from enterotoxigenic Escherichia coli genomic DNA that carry Rns binding sites. In vivo transcription fusion analysis shows that Rns positively regulates the expression of the open reading frame of yiiS, which lies immediately downstream of one MBP-Rns-bound fragment.

Original languageEnglish (US)
Pages (from-to)1196-1199
Number of pages4
JournalJournal of bacteriology
Issue number4
StatePublished - 2002
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology


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