In vitro identification of Rns-regulated genes

George P. Munson, Lisa G. Holcomb, Heather L. Alexander, June R. Scott

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

To identify Rns-regulated genes, a maltose binding protein (MBP)-Rns fusion protein was used to isolate DNA fragments from enterotoxigenic Escherichia coli genomic DNA that carry Rns binding sites. In vivo transcription fusion analysis shows that Rns positively regulates the expression of the open reading frame of yiiS, which lies immediately downstream of one MBP-Rns-bound fragment.

Original languageEnglish (US)
Pages (from-to)1196-1199
Number of pages4
JournalJournal of bacteriology
Volume184
Issue number4
DOIs
StatePublished - 2002

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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