In vitro global gene expression analyses support the ethnopharmacological use of Achyranthes aspera

Subbayan Pochi, Malancha Sarkar, Lubov Nathanson, Nikesh Doshi, Balakrishna L. Lokeshwar, Bach Ardalan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Achyranthes aspera (family Amaranthaceae) is known for its anticancer properties. We have systematically validated the in vitro and in vivo anticancer properties of this plant. However, we do not know its mode of action. Global gene expression analyses may help decipher its mode of action. In the absence of identified active molecules, we believe this is the best approach to discover the mode of action of natural products with known medicinal properties. We exposed human pancreatic cancer cell line MiaPaCa-2 (CRL-1420) to 34 g/mL of LE for 24, 48, and 72 hours. Gene expression analyses were performed using whole human genome microarrays (Agilent Technologies, USA). In our analyses, 82 (54/28) genes passed the quality control parameter, set at FDR ≤ 0.01 and FC of ≥±2. LE predominantly affected pathways of immune response, metabolism, development, gene expression regulation, cell adhesion, cystic fibrosis transmembrane conductance regulation (CFTR), and chemotaxis (MetaCore tool (Thomson Reuters, NY)). Disease biomarker enrichment analysis identified LE regulated genes involved in Vasculitis - inflammation of blood vessels. Arthritis and pancreatitis are two of many etiologies for vasculitis. The outcome of disease network analysis supports the medicinal use of A. aspera, viz, to stop bleeding, as a cure for pancreatic cancer, as an antiarthritic medication, and so forth.

Original languageEnglish
Article number471739
JournalEvidence-based Complementary and Alternative Medicine
Volume2013
DOIs
StatePublished - Dec 1 2013

Fingerprint

Achyranthes
Vasculitis
Pancreatic Neoplasms
Amaranthaceae
Gene Expression
Gene Expression Regulation
Human Genome
Chemotaxis
Biological Products
Cystic Fibrosis
Cell Adhesion
Quality Control
Pancreatitis
Genes
Arthritis
Blood Vessels
Biomarkers
Hemorrhage
Inflammation
Technology

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

In vitro global gene expression analyses support the ethnopharmacological use of Achyranthes aspera. / Pochi, Subbayan; Sarkar, Malancha; Nathanson, Lubov; Doshi, Nikesh; Lokeshwar, Balakrishna L.; Ardalan, Bach.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2013, 471739, 01.12.2013.

Research output: Contribution to journalArticle

Pochi, Subbayan ; Sarkar, Malancha ; Nathanson, Lubov ; Doshi, Nikesh ; Lokeshwar, Balakrishna L. ; Ardalan, Bach. / In vitro global gene expression analyses support the ethnopharmacological use of Achyranthes aspera. In: Evidence-based Complementary and Alternative Medicine. 2013 ; Vol. 2013.
@article{8535e8b629954890be8317bf28c22b43,
title = "In vitro global gene expression analyses support the ethnopharmacological use of Achyranthes aspera",
abstract = "Achyranthes aspera (family Amaranthaceae) is known for its anticancer properties. We have systematically validated the in vitro and in vivo anticancer properties of this plant. However, we do not know its mode of action. Global gene expression analyses may help decipher its mode of action. In the absence of identified active molecules, we believe this is the best approach to discover the mode of action of natural products with known medicinal properties. We exposed human pancreatic cancer cell line MiaPaCa-2 (CRL-1420) to 34 g/mL of LE for 24, 48, and 72 hours. Gene expression analyses were performed using whole human genome microarrays (Agilent Technologies, USA). In our analyses, 82 (54/28) genes passed the quality control parameter, set at FDR ≤ 0.01 and FC of ≥±2. LE predominantly affected pathways of immune response, metabolism, development, gene expression regulation, cell adhesion, cystic fibrosis transmembrane conductance regulation (CFTR), and chemotaxis (MetaCore tool (Thomson Reuters, NY)). Disease biomarker enrichment analysis identified LE regulated genes involved in Vasculitis - inflammation of blood vessels. Arthritis and pancreatitis are two of many etiologies for vasculitis. The outcome of disease network analysis supports the medicinal use of A. aspera, viz, to stop bleeding, as a cure for pancreatic cancer, as an antiarthritic medication, and so forth.",
author = "Subbayan Pochi and Malancha Sarkar and Lubov Nathanson and Nikesh Doshi and Lokeshwar, {Balakrishna L.} and Bach Ardalan",
year = "2013",
month = "12",
day = "1",
doi = "10.1155/2013/471739",
language = "English",
volume = "2013",
journal = "Evidence-based Complementary and Alternative Medicine",
issn = "1741-427X",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - In vitro global gene expression analyses support the ethnopharmacological use of Achyranthes aspera

AU - Pochi, Subbayan

AU - Sarkar, Malancha

AU - Nathanson, Lubov

AU - Doshi, Nikesh

AU - Lokeshwar, Balakrishna L.

AU - Ardalan, Bach

PY - 2013/12/1

Y1 - 2013/12/1

N2 - Achyranthes aspera (family Amaranthaceae) is known for its anticancer properties. We have systematically validated the in vitro and in vivo anticancer properties of this plant. However, we do not know its mode of action. Global gene expression analyses may help decipher its mode of action. In the absence of identified active molecules, we believe this is the best approach to discover the mode of action of natural products with known medicinal properties. We exposed human pancreatic cancer cell line MiaPaCa-2 (CRL-1420) to 34 g/mL of LE for 24, 48, and 72 hours. Gene expression analyses were performed using whole human genome microarrays (Agilent Technologies, USA). In our analyses, 82 (54/28) genes passed the quality control parameter, set at FDR ≤ 0.01 and FC of ≥±2. LE predominantly affected pathways of immune response, metabolism, development, gene expression regulation, cell adhesion, cystic fibrosis transmembrane conductance regulation (CFTR), and chemotaxis (MetaCore tool (Thomson Reuters, NY)). Disease biomarker enrichment analysis identified LE regulated genes involved in Vasculitis - inflammation of blood vessels. Arthritis and pancreatitis are two of many etiologies for vasculitis. The outcome of disease network analysis supports the medicinal use of A. aspera, viz, to stop bleeding, as a cure for pancreatic cancer, as an antiarthritic medication, and so forth.

AB - Achyranthes aspera (family Amaranthaceae) is known for its anticancer properties. We have systematically validated the in vitro and in vivo anticancer properties of this plant. However, we do not know its mode of action. Global gene expression analyses may help decipher its mode of action. In the absence of identified active molecules, we believe this is the best approach to discover the mode of action of natural products with known medicinal properties. We exposed human pancreatic cancer cell line MiaPaCa-2 (CRL-1420) to 34 g/mL of LE for 24, 48, and 72 hours. Gene expression analyses were performed using whole human genome microarrays (Agilent Technologies, USA). In our analyses, 82 (54/28) genes passed the quality control parameter, set at FDR ≤ 0.01 and FC of ≥±2. LE predominantly affected pathways of immune response, metabolism, development, gene expression regulation, cell adhesion, cystic fibrosis transmembrane conductance regulation (CFTR), and chemotaxis (MetaCore tool (Thomson Reuters, NY)). Disease biomarker enrichment analysis identified LE regulated genes involved in Vasculitis - inflammation of blood vessels. Arthritis and pancreatitis are two of many etiologies for vasculitis. The outcome of disease network analysis supports the medicinal use of A. aspera, viz, to stop bleeding, as a cure for pancreatic cancer, as an antiarthritic medication, and so forth.

UR - http://www.scopus.com/inward/record.url?scp=84893771313&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893771313&partnerID=8YFLogxK

U2 - 10.1155/2013/471739

DO - 10.1155/2013/471739

M3 - Article

AN - SCOPUS:84893771313

VL - 2013

JO - Evidence-based Complementary and Alternative Medicine

JF - Evidence-based Complementary and Alternative Medicine

SN - 1741-427X

M1 - 471739

ER -