In vitro generation of three-dimensional renal structures

Akira Joraku, Kathryn A. Stern, Anthony Atala, James J. Yoo

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

End-stage renal disease is currently being treated effectively by transplantation. However, increasing demand and donor shortage make this treatment challenging. Recent advances in cell-based therapies have provided potential opportunities to alleviate the current challenges of donor shortage. In this study we developed a system to generate renal structures in vitro using primary kidney cells. This system involves the cultivation of expanded primary renal cells in a three-dimensional collagen-based culture system. After one week of growth, individual renal cells began to form renal structures resembling tubules and glomeruli. Histologically, these structures show phenotypic resemblance to native kidney structures. The reconstituted tubules stained positively for Tamm-Horsfall protein, which is expressed in the thick ascending limb of Henle's Loop and distal convoluted tubules. These results show that renal structures can be reconstituted in a three-dimensional culture system, which may eventually be used for renal cell therapy applications.

Original languageEnglish
Pages (from-to)129-133
Number of pages5
JournalMethods
Volume47
Issue number2
DOIs
StatePublished - Feb 1 2009
Externally publishedYes

Fingerprint

Uromodulin
Collagen
Kidney
Cell- and Tissue-Based Therapy
Loop of Henle
In Vitro Techniques
Chronic Kidney Failure
Transplantation

Keywords

  • Cell-based therapy
  • Collagen
  • End-stage renal disease
  • Glomeruli
  • Renal structures
  • Renal tubules

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Joraku, A., Stern, K. A., Atala, A., & Yoo, J. J. (2009). In vitro generation of three-dimensional renal structures. Methods, 47(2), 129-133. https://doi.org/10.1016/j.ymeth.2008.09.005

In vitro generation of three-dimensional renal structures. / Joraku, Akira; Stern, Kathryn A.; Atala, Anthony; Yoo, James J.

In: Methods, Vol. 47, No. 2, 01.02.2009, p. 129-133.

Research output: Contribution to journalArticle

Joraku, A, Stern, KA, Atala, A & Yoo, JJ 2009, 'In vitro generation of three-dimensional renal structures', Methods, vol. 47, no. 2, pp. 129-133. https://doi.org/10.1016/j.ymeth.2008.09.005
Joraku, Akira ; Stern, Kathryn A. ; Atala, Anthony ; Yoo, James J. / In vitro generation of three-dimensional renal structures. In: Methods. 2009 ; Vol. 47, No. 2. pp. 129-133.
@article{2a734bf4c71b4435bad9194944478d1a,
title = "In vitro generation of three-dimensional renal structures",
abstract = "End-stage renal disease is currently being treated effectively by transplantation. However, increasing demand and donor shortage make this treatment challenging. Recent advances in cell-based therapies have provided potential opportunities to alleviate the current challenges of donor shortage. In this study we developed a system to generate renal structures in vitro using primary kidney cells. This system involves the cultivation of expanded primary renal cells in a three-dimensional collagen-based culture system. After one week of growth, individual renal cells began to form renal structures resembling tubules and glomeruli. Histologically, these structures show phenotypic resemblance to native kidney structures. The reconstituted tubules stained positively for Tamm-Horsfall protein, which is expressed in the thick ascending limb of Henle's Loop and distal convoluted tubules. These results show that renal structures can be reconstituted in a three-dimensional culture system, which may eventually be used for renal cell therapy applications.",
keywords = "Cell-based therapy, Collagen, End-stage renal disease, Glomeruli, Renal structures, Renal tubules",
author = "Akira Joraku and Stern, {Kathryn A.} and Anthony Atala and Yoo, {James J.}",
year = "2009",
month = "2",
day = "1",
doi = "10.1016/j.ymeth.2008.09.005",
language = "English",
volume = "47",
pages = "129--133",
journal = "Methods in Enzymology",
issn = "0076-6879",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - In vitro generation of three-dimensional renal structures

AU - Joraku, Akira

AU - Stern, Kathryn A.

AU - Atala, Anthony

AU - Yoo, James J.

PY - 2009/2/1

Y1 - 2009/2/1

N2 - End-stage renal disease is currently being treated effectively by transplantation. However, increasing demand and donor shortage make this treatment challenging. Recent advances in cell-based therapies have provided potential opportunities to alleviate the current challenges of donor shortage. In this study we developed a system to generate renal structures in vitro using primary kidney cells. This system involves the cultivation of expanded primary renal cells in a three-dimensional collagen-based culture system. After one week of growth, individual renal cells began to form renal structures resembling tubules and glomeruli. Histologically, these structures show phenotypic resemblance to native kidney structures. The reconstituted tubules stained positively for Tamm-Horsfall protein, which is expressed in the thick ascending limb of Henle's Loop and distal convoluted tubules. These results show that renal structures can be reconstituted in a three-dimensional culture system, which may eventually be used for renal cell therapy applications.

AB - End-stage renal disease is currently being treated effectively by transplantation. However, increasing demand and donor shortage make this treatment challenging. Recent advances in cell-based therapies have provided potential opportunities to alleviate the current challenges of donor shortage. In this study we developed a system to generate renal structures in vitro using primary kidney cells. This system involves the cultivation of expanded primary renal cells in a three-dimensional collagen-based culture system. After one week of growth, individual renal cells began to form renal structures resembling tubules and glomeruli. Histologically, these structures show phenotypic resemblance to native kidney structures. The reconstituted tubules stained positively for Tamm-Horsfall protein, which is expressed in the thick ascending limb of Henle's Loop and distal convoluted tubules. These results show that renal structures can be reconstituted in a three-dimensional culture system, which may eventually be used for renal cell therapy applications.

KW - Cell-based therapy

KW - Collagen

KW - End-stage renal disease

KW - Glomeruli

KW - Renal structures

KW - Renal tubules

UR - http://www.scopus.com/inward/record.url?scp=58049219896&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58049219896&partnerID=8YFLogxK

U2 - 10.1016/j.ymeth.2008.09.005

DO - 10.1016/j.ymeth.2008.09.005

M3 - Article

C2 - 18845258

AN - SCOPUS:58049219896

VL - 47

SP - 129

EP - 133

JO - Methods in Enzymology

JF - Methods in Enzymology

SN - 0076-6879

IS - 2

ER -