In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region

Yoh Suke Mukouyama, Takahiko Hara, Mingjiang Xu, Kazuhiro Tamura, Peter J. Donovan, Hee Jung Kim, Hiroshi Kogo, Kohichiro Tsuji, Tatsutoshi Nakahata, Atsushi Miyajima

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

The origin of hematopoietic stem cells (HSCs) and their growth factor requirement are poorly understood. Here we describe a new in vitro culture system of the aorta-gonad-mesonephros (AGM) region, where long-term repopulating HSCs first arise. We demonstrate that oncostatin M (OSM) is expressed in the AGM and is absolutely required for the expansion of multipotential hematopoietic progenitors in vitro. In addition, OSM enhances the formation of endothelial cell clusters. Thus, OSM appears to be a key cytokine for the development of multipotential hematopoietic progenitors in the AGM, possibly acting on common precursor cells between HSCs and endothelial cells. By using the AGM culture derived from macrophage colony- stimulating factor (M-CSF)-deficient op/op mutant embryos, we also show a pivotal role for M-CSF in fetal myelopoiesis.

Original languageEnglish (US)
Pages (from-to)105-114
Number of pages10
JournalImmunity
Volume8
Issue number1
DOIs
StatePublished - Jan 1998
Externally publishedYes

Fingerprint

Mesonephros
Oncostatin M
Gonads
Aorta
Hematopoietic Stem Cells
Macrophage Colony-Stimulating Factor
Endothelial Cells
Myelopoiesis
Intercellular Signaling Peptides and Proteins
Embryonic Structures
Cytokines
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region. / Mukouyama, Yoh Suke; Hara, Takahiko; Xu, Mingjiang; Tamura, Kazuhiro; Donovan, Peter J.; Kim, Hee Jung; Kogo, Hiroshi; Tsuji, Kohichiro; Nakahata, Tatsutoshi; Miyajima, Atsushi.

In: Immunity, Vol. 8, No. 1, 01.1998, p. 105-114.

Research output: Contribution to journalArticle

Mukouyama, YS, Hara, T, Xu, M, Tamura, K, Donovan, PJ, Kim, HJ, Kogo, H, Tsuji, K, Nakahata, T & Miyajima, A 1998, 'In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region', Immunity, vol. 8, no. 1, pp. 105-114. https://doi.org/10.1016/S1074-7613(00)80463-X
Mukouyama, Yoh Suke ; Hara, Takahiko ; Xu, Mingjiang ; Tamura, Kazuhiro ; Donovan, Peter J. ; Kim, Hee Jung ; Kogo, Hiroshi ; Tsuji, Kohichiro ; Nakahata, Tatsutoshi ; Miyajima, Atsushi. / In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region. In: Immunity. 1998 ; Vol. 8, No. 1. pp. 105-114.
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