Abstract
The origin of hematopoietic stem cells (HSCs) and their growth factor requirement are poorly understood. Here we describe a new in vitro culture system of the aorta-gonad-mesonephros (AGM) region, where long-term repopulating HSCs first arise. We demonstrate that oncostatin M (OSM) is expressed in the AGM and is absolutely required for the expansion of multipotential hematopoietic progenitors in vitro. In addition, OSM enhances the formation of endothelial cell clusters. Thus, OSM appears to be a key cytokine for the development of multipotential hematopoietic progenitors in the AGM, possibly acting on common precursor cells between HSCs and endothelial cells. By using the AGM culture derived from macrophage colony- stimulating factor (M-CSF)-deficient op/op mutant embryos, we also show a pivotal role for M-CSF in fetal myelopoiesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 105-114 |
| Number of pages | 10 |
| Journal | Immunity |
| Volume | 8 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology and Allergy
- Infectious Diseases
- Immunology
Cite this
In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region. / Mukouyama, Yoh Suke; Hara, Takahiko; Xu, Mingjiang; Tamura, Kazuhiro; Donovan, Peter J.; Kim, Hee Jung; Kogo, Hiroshi; Tsuji, Kohichiro; Nakahata, Tatsutoshi; Miyajima, Atsushi.
In: Immunity, Vol. 8, No. 1, 01.1998, p. 105-114.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region
AU - Mukouyama, Yoh Suke
AU - Hara, Takahiko
AU - Xu, Mingjiang
AU - Tamura, Kazuhiro
AU - Donovan, Peter J.
AU - Kim, Hee Jung
AU - Kogo, Hiroshi
AU - Tsuji, Kohichiro
AU - Nakahata, Tatsutoshi
AU - Miyajima, Atsushi
PY - 1998/1
Y1 - 1998/1
N2 - The origin of hematopoietic stem cells (HSCs) and their growth factor requirement are poorly understood. Here we describe a new in vitro culture system of the aorta-gonad-mesonephros (AGM) region, where long-term repopulating HSCs first arise. We demonstrate that oncostatin M (OSM) is expressed in the AGM and is absolutely required for the expansion of multipotential hematopoietic progenitors in vitro. In addition, OSM enhances the formation of endothelial cell clusters. Thus, OSM appears to be a key cytokine for the development of multipotential hematopoietic progenitors in the AGM, possibly acting on common precursor cells between HSCs and endothelial cells. By using the AGM culture derived from macrophage colony- stimulating factor (M-CSF)-deficient op/op mutant embryos, we also show a pivotal role for M-CSF in fetal myelopoiesis.
AB - The origin of hematopoietic stem cells (HSCs) and their growth factor requirement are poorly understood. Here we describe a new in vitro culture system of the aorta-gonad-mesonephros (AGM) region, where long-term repopulating HSCs first arise. We demonstrate that oncostatin M (OSM) is expressed in the AGM and is absolutely required for the expansion of multipotential hematopoietic progenitors in vitro. In addition, OSM enhances the formation of endothelial cell clusters. Thus, OSM appears to be a key cytokine for the development of multipotential hematopoietic progenitors in the AGM, possibly acting on common precursor cells between HSCs and endothelial cells. By using the AGM culture derived from macrophage colony- stimulating factor (M-CSF)-deficient op/op mutant embryos, we also show a pivotal role for M-CSF in fetal myelopoiesis.
UR - http://www.scopus.com/inward/record.url?scp=0031951550&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031951550&partnerID=8YFLogxK
U2 - 10.1016/S1074-7613(00)80463-X
DO - 10.1016/S1074-7613(00)80463-X
M3 - Article
C2 - 9462516
AN - SCOPUS:0031951550
VL - 8
SP - 105
EP - 114
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 1
ER -