In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region

Yoh Suke Mukouyama; Takahiko Hara; Ming Jiang Xu; Kazuhiro Tamura; Peter J. Donovan; Hee Jung Kim; Hiroshi Kogo; Kohichiro Tsuji; Tatsutoshi Nakahata; Atsushi Miyajima

doi:10.1016/S1074-7613(00)80463-X

In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region

Yoh Suke Mukouyama, Takahiko Hara, Ming Jiang Xu, Kazuhiro Tamura, Peter J. Donovan, Hee Jung Kim, Hiroshi Kogo, Kohichiro Tsuji, Tatsutoshi Nakahata, Atsushi Miyajima

Research output: Contribution to journal › Article

118 Scopus citations

Abstract

The origin of hematopoietic stem cells (HSCs) and their growth factor requirement are poorly understood. Here we describe a new in vitro culture system of the aorta-gonad-mesonephros (AGM) region, where long-term repopulating HSCs first arise. We demonstrate that oncostatin M (OSM) is expressed in the AGM and is absolutely required for the expansion of multipotential hematopoietic progenitors in vitro. In addition, OSM enhances the formation of endothelial cell clusters. Thus, OSM appears to be a key cytokine for the development of multipotential hematopoietic progenitors in the AGM, possibly acting on common precursor cells between HSCs and endothelial cells. By using the AGM culture derived from macrophage colony- stimulating factor (M-CSF)-deficient op/op mutant embryos, we also show a pivotal role for M-CSF in fetal myelopoiesis.

Original language	English (US)
Pages (from-to)	105-114
Number of pages	10
Journal	Immunity
Volume	8
Issue number	1
DOIs	https://doi.org/10.1016/S1074-7613(00)80463-X
State	Published - Jan 1998
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Infectious Diseases

Access to Document

10.1016/S1074-7613(00)80463-X

Fingerprint Dive into the research topics of 'In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region'. Together they form a unique fingerprint.

Cite this

Mukouyama, Y. S., Hara, T., Xu, M. J., Tamura, K., Donovan, P. J., Kim, H. J., Kogo, H., Tsuji, K., Nakahata, T., & Miyajima, A. (1998). In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region. Immunity, 8(1), 105-114. https://doi.org/10.1016/S1074-7613(00)80463-X

In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region. / Mukouyama, Yoh Suke; Hara, Takahiko; Xu, Ming Jiang; Tamura, Kazuhiro; Donovan, Peter J.; Kim, Hee Jung; Kogo, Hiroshi; Tsuji, Kohichiro; Nakahata, Tatsutoshi; Miyajima, Atsushi.

In: Immunity, Vol. 8, No. 1, 01.1998, p. 105-114.

Research output: Contribution to journal › Article

@article{00ab24df662c454bbc370974c30e664a,

title = "In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region",

abstract = "The origin of hematopoietic stem cells (HSCs) and their growth factor requirement are poorly understood. Here we describe a new in vitro culture system of the aorta-gonad-mesonephros (AGM) region, where long-term repopulating HSCs first arise. We demonstrate that oncostatin M (OSM) is expressed in the AGM and is absolutely required for the expansion of multipotential hematopoietic progenitors in vitro. In addition, OSM enhances the formation of endothelial cell clusters. Thus, OSM appears to be a key cytokine for the development of multipotential hematopoietic progenitors in the AGM, possibly acting on common precursor cells between HSCs and endothelial cells. By using the AGM culture derived from macrophage colony- stimulating factor (M-CSF)-deficient op/op mutant embryos, we also show a pivotal role for M-CSF in fetal myelopoiesis.",

author = "Mukouyama, {Yoh Suke} and Takahiko Hara and Xu, {Ming Jiang} and Kazuhiro Tamura and Donovan, {Peter J.} and Kim, {Hee Jung} and Hiroshi Kogo and Kohichiro Tsuji and Tatsutoshi Nakahata and Atsushi Miyajima",

year = "1998",

month = jan,

doi = "10.1016/S1074-7613(00)80463-X",

language = "English (US)",

volume = "8",

pages = "105--114",

journal = "Immunity",

issn = "1074-7613",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region

AU - Mukouyama, Yoh Suke

AU - Hara, Takahiko

AU - Xu, Ming Jiang

AU - Tamura, Kazuhiro

AU - Donovan, Peter J.

AU - Kim, Hee Jung

AU - Kogo, Hiroshi

AU - Tsuji, Kohichiro

AU - Nakahata, Tatsutoshi

AU - Miyajima, Atsushi

PY - 1998/1

Y1 - 1998/1

N2 - The origin of hematopoietic stem cells (HSCs) and their growth factor requirement are poorly understood. Here we describe a new in vitro culture system of the aorta-gonad-mesonephros (AGM) region, where long-term repopulating HSCs first arise. We demonstrate that oncostatin M (OSM) is expressed in the AGM and is absolutely required for the expansion of multipotential hematopoietic progenitors in vitro. In addition, OSM enhances the formation of endothelial cell clusters. Thus, OSM appears to be a key cytokine for the development of multipotential hematopoietic progenitors in the AGM, possibly acting on common precursor cells between HSCs and endothelial cells. By using the AGM culture derived from macrophage colony- stimulating factor (M-CSF)-deficient op/op mutant embryos, we also show a pivotal role for M-CSF in fetal myelopoiesis.

AB - The origin of hematopoietic stem cells (HSCs) and their growth factor requirement are poorly understood. Here we describe a new in vitro culture system of the aorta-gonad-mesonephros (AGM) region, where long-term repopulating HSCs first arise. We demonstrate that oncostatin M (OSM) is expressed in the AGM and is absolutely required for the expansion of multipotential hematopoietic progenitors in vitro. In addition, OSM enhances the formation of endothelial cell clusters. Thus, OSM appears to be a key cytokine for the development of multipotential hematopoietic progenitors in the AGM, possibly acting on common precursor cells between HSCs and endothelial cells. By using the AGM culture derived from macrophage colony- stimulating factor (M-CSF)-deficient op/op mutant embryos, we also show a pivotal role for M-CSF in fetal myelopoiesis.

UR - http://www.scopus.com/inward/record.url?scp=0031951550&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031951550&partnerID=8YFLogxK

U2 - 10.1016/S1074-7613(00)80463-X

DO - 10.1016/S1074-7613(00)80463-X

M3 - Article

C2 - 9462516

AN - SCOPUS:0031951550

VL - 8

SP - 105

EP - 114

JO - Immunity

JF - Immunity

SN - 1074-7613

IS - 1

ER -