In vitro evidence for T-B lymphocyte collaboration in the clawed toad, Xenopus

Bonnie B Blomberg, C. C A Bernard, L. Du Pasquier

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Requirements for an in vitro secondary antibody response to the soluble antigens dinitrophenylated keyhole limpet hemocyanin and dinitrophenylated fowl gamma-globulin are described for the clawed toad, Xenopus. Priming of both hapten and carrier-specific cells is required in order to obtain good responses to the hapten. The carrier-reactive ('helper') cells do not adhere to nylon wool, are X ray-resistant and surface Ig-negative. The cell adherence and X ray sensitivity characteristics of these Xenopus 'T' cells and their ability to generate a mixed lymphocyte response, allogeneic cytotoxic and helper activities, are identical to those of thymus-derived (T) cells in higher vertebrates. The hapten-reactive, antibody-secreting cell precursors are nylon-adherent, X ray-sensitive and surface Ig-positive. In the absence of other distinguishing serological markers for T cells in frogs, and considering the thymus dependency of the helper activity described here, the evidence presented is interpreted as demonstrating the requirement for antigen-specific T-B cell cooperation in response to soluble protein antigens in vitro for Xenopus.

Original languageEnglish
Pages (from-to)869-876
Number of pages8
JournalEuropean Journal of Immunology
Volume10
Issue number11
StatePublished - Dec 1 1980
Externally publishedYes

Fingerprint

Haptens
Xenopus
Anura
B-Lymphocytes
Nylons
X-Rays
T-Lymphocytes
Thymus Gland
Antigens
Antibody-Producing Cells
Wool
gamma-Globulins
Viral Tumor Antigens
Helper-Inducer T-Lymphocytes
Antibody Formation
Vertebrates
Lymphocytes
In Vitro Techniques
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

In vitro evidence for T-B lymphocyte collaboration in the clawed toad, Xenopus. / Blomberg, Bonnie B; Bernard, C. C A; Du Pasquier, L.

In: European Journal of Immunology, Vol. 10, No. 11, 01.12.1980, p. 869-876.

Research output: Contribution to journalArticle

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