In vitro evaluation of electrospun nanofiber scaffolds for vascular graft application

Sang Jin Lee, James J. Yoo, Grace J. Lim, Anthony Atala, Joel Stitzel

Research output: Contribution to journalArticle

223 Scopus citations

Abstract

Blood vessels are diverse in size, mechanical and biochemical properties, cellular content, and ultrastructural organization depending on their location and specific function. Therefore, it is required to control the fabrication of vascular grafts for obtaining desirable characteristics of blood vessel substitutes. In this study we have fabricated various scaffolds using the electrospinning technique with blends of collagen, elastin, and several biodegradable polymers. Biocompatibility, dimensional stability in vitro and mechanical properties were evaluated. Materials were blended at a relative concentration by weight of 45% collagen, 15% elastin, and 40% synthetic polymer to mimic the ratio of collagen and elastin in native blood vessels. The fabricated scaffolds are composed of randomly oriented fibers with diameters ranging from 477 to 765 nm. The electrospun scaffolds are non-toxic, dimensionally stable in an in vitro culture environment, easily fabricated, and possess controlled mechanical properties that simulate the ultrastructure of native blood vessels. The present study suggests that the introduction of synthetic biodegradable polymers enabled tailoring of mechanical properties of vascular substitutes and improving compliance matching for vascular tissue engineering.

Original languageEnglish (US)
Pages (from-to)999-1008
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume83
Issue number4
DOIs
StatePublished - Dec 15 2007

Keywords

  • Biomimetic
  • Collagen
  • Elastin
  • Electrospinning
  • Tissue engineering

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

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