In vitro cardiomyogenic potential of human amniotic fluid stem cells

Xuan Guan, Dawn M. Delo, Anthony Atala, Shay Soker

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Stem cell therapy for damaged cardiac tissue is currently limited by a number of factors, including inability to obtain sufficient cell numbers, the potential tumorigenicity of certain types of stem cells and the possible link between stem cell therapy and the development of malignant arrhythmias. In this study, we investigated whether human amniotic fluid-derived stem (hAFS) cells could be a potential source of cells for cardiac cell therapy, by testing the in vitro differentiation capabilities. Undifferentiated hAFS cells express several cardiac genes, including the transcription factor mef2, the gap junction connexin43, and H- and N-cadherin. A 24 h incubation with 5-aza-2'-deoxycytidine (5-AZA-dC) induced hAFS cell differentiation along the cardiac lineage. Evidence for this differentiation included morphological changes, upregulation of cardiac-specific genes (cardiac troponin I and cardiac troponin T) and redistribution of connexin43, as well as downregulation of the stem cell marker SRY-box 2 (sox2). When co-cultured with neonatal rat cardiomyocytes (NRCs), hAFS cells formed both mechanical and electrical connections with the NRCs. Dye transfer experiments showed that calcein dye could be transferred from NRCs to hAFS cells through cellular connections. The gap junction connexin43 likely involved in the communication between the two cell types, because 12-O-tetradecanoylphorbol 13-acetate (TPA) could partially block cellular crosstalk. We conclude that hAFS cells can be differentiated into a cardiomyocyte-like phenotype and can establish functional communication with NRCs. Thus, hAFS cells may potentially be used for cardiac cell therapy.

Original languageEnglish
Pages (from-to)220-228
Number of pages9
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume5
Issue number3
DOIs
StatePublished - Mar 1 2011
Externally publishedYes

Fingerprint

Amniotic Fluid
Stem cells
Stem Cells
Fluids
Cardiac Myocytes
Cell- and Tissue-Based Therapy
Connexin 43
Rats
Gap Junctions
decitabine
In Vitro Techniques
Coloring Agents
Dyes
Genes
Communication
Troponin T
Troponin I
Transcription factors
Tetradecanoylphorbol Acetate
Cadherins

Keywords

  • Amniotic fluid stem cells
  • Cardiac
  • Cell differentiation
  • Co-culture techniques
  • Myocytes
  • Stem cells

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials

Cite this

In vitro cardiomyogenic potential of human amniotic fluid stem cells. / Guan, Xuan; Delo, Dawn M.; Atala, Anthony; Soker, Shay.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 5, No. 3, 01.03.2011, p. 220-228.

Research output: Contribution to journalArticle

Guan, Xuan ; Delo, Dawn M. ; Atala, Anthony ; Soker, Shay. / In vitro cardiomyogenic potential of human amniotic fluid stem cells. In: Journal of Tissue Engineering and Regenerative Medicine. 2011 ; Vol. 5, No. 3. pp. 220-228.
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