In vitro biocompatibility assessment of naturally derived and synthetic biomaterials using normal human urothelial cells

Jean Louis Pariente, Byung Soo Kim, Anthony Atala

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

The reconstruction of urinary tissues often employs various types of biomaterials, and adequate material biocompatibility is essential for the successful reconstruction of urinary tissues. In this study we utilized a primary normal human urothelial cell culture system to evaluate the in vitro biocompatibility of a number of naturally derived biomaterials [i.e., bladder submucosa, small intestinal submucosa, collagen, and alginate] and polymeric biomaterials [i.e., poly(glycolic acid), poly(L-lactic acid), poly(lactic-co-glycolic acid), and silicone] that are either experimentally or clinically used in urinary reconstructive surgery. To determine the cytotoxic and bioactive effects of these biomaterials, the cell viability, metabolic activity, apoptotic properties, and DNA-synthesis activity were measured with four types of assays [Neutral Red, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, apoptotic activity, and tritiated thymidine incorporation assays] using extract and direct contact methods. Most of the biomaterials tested did not induce significant cytotoxic effects and exhibited normal metabolic function and cell growth in vitro. This normal primary human urothelial cell culture model is suitable for in vitro biocompatibility assessments and is able to provide information on the cell-biomaterial interactions and the ability of biomaterials to support bioactive cell functions.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalJournal of Biomedical Materials Research
Volume55
Issue number1
DOIs
StatePublished - Feb 27 2001
Externally publishedYes

Fingerprint

Biocompatible Materials
Biocompatibility
Biomaterials
glycolic acid
Cell culture
Assays
Tissue
Neutral Red
Acids
Alginate
Cell growth
Silicones
Lactic acid
Collagen
Thymidine
Surgery
DNA
Cells

Keywords

  • Biocompatibility
  • Natural biomaterials
  • Synthetic polymers
  • Urothelial cells

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

In vitro biocompatibility assessment of naturally derived and synthetic biomaterials using normal human urothelial cells. / Pariente, Jean Louis; Kim, Byung Soo; Atala, Anthony.

In: Journal of Biomedical Materials Research, Vol. 55, No. 1, 27.02.2001, p. 33-39.

Research output: Contribution to journalArticle

@article{0fd74ccde0b44fa79e857533390b5b85,
title = "In vitro biocompatibility assessment of naturally derived and synthetic biomaterials using normal human urothelial cells",
abstract = "The reconstruction of urinary tissues often employs various types of biomaterials, and adequate material biocompatibility is essential for the successful reconstruction of urinary tissues. In this study we utilized a primary normal human urothelial cell culture system to evaluate the in vitro biocompatibility of a number of naturally derived biomaterials [i.e., bladder submucosa, small intestinal submucosa, collagen, and alginate] and polymeric biomaterials [i.e., poly(glycolic acid), poly(L-lactic acid), poly(lactic-co-glycolic acid), and silicone] that are either experimentally or clinically used in urinary reconstructive surgery. To determine the cytotoxic and bioactive effects of these biomaterials, the cell viability, metabolic activity, apoptotic properties, and DNA-synthesis activity were measured with four types of assays [Neutral Red, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, apoptotic activity, and tritiated thymidine incorporation assays] using extract and direct contact methods. Most of the biomaterials tested did not induce significant cytotoxic effects and exhibited normal metabolic function and cell growth in vitro. This normal primary human urothelial cell culture model is suitable for in vitro biocompatibility assessments and is able to provide information on the cell-biomaterial interactions and the ability of biomaterials to support bioactive cell functions.",
keywords = "Biocompatibility, Natural biomaterials, Synthetic polymers, Urothelial cells",
author = "Pariente, {Jean Louis} and Kim, {Byung Soo} and Anthony Atala",
year = "2001",
month = "2",
day = "27",
doi = "10.1002/1097-4636(200104)55:1<33::AID-JBM50>3.0.CO;2-7",
language = "English",
volume = "55",
pages = "33--39",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "1549-3296",
publisher = "Heterocorporation",
number = "1",

}

TY - JOUR

T1 - In vitro biocompatibility assessment of naturally derived and synthetic biomaterials using normal human urothelial cells

AU - Pariente, Jean Louis

AU - Kim, Byung Soo

AU - Atala, Anthony

PY - 2001/2/27

Y1 - 2001/2/27

N2 - The reconstruction of urinary tissues often employs various types of biomaterials, and adequate material biocompatibility is essential for the successful reconstruction of urinary tissues. In this study we utilized a primary normal human urothelial cell culture system to evaluate the in vitro biocompatibility of a number of naturally derived biomaterials [i.e., bladder submucosa, small intestinal submucosa, collagen, and alginate] and polymeric biomaterials [i.e., poly(glycolic acid), poly(L-lactic acid), poly(lactic-co-glycolic acid), and silicone] that are either experimentally or clinically used in urinary reconstructive surgery. To determine the cytotoxic and bioactive effects of these biomaterials, the cell viability, metabolic activity, apoptotic properties, and DNA-synthesis activity were measured with four types of assays [Neutral Red, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, apoptotic activity, and tritiated thymidine incorporation assays] using extract and direct contact methods. Most of the biomaterials tested did not induce significant cytotoxic effects and exhibited normal metabolic function and cell growth in vitro. This normal primary human urothelial cell culture model is suitable for in vitro biocompatibility assessments and is able to provide information on the cell-biomaterial interactions and the ability of biomaterials to support bioactive cell functions.

AB - The reconstruction of urinary tissues often employs various types of biomaterials, and adequate material biocompatibility is essential for the successful reconstruction of urinary tissues. In this study we utilized a primary normal human urothelial cell culture system to evaluate the in vitro biocompatibility of a number of naturally derived biomaterials [i.e., bladder submucosa, small intestinal submucosa, collagen, and alginate] and polymeric biomaterials [i.e., poly(glycolic acid), poly(L-lactic acid), poly(lactic-co-glycolic acid), and silicone] that are either experimentally or clinically used in urinary reconstructive surgery. To determine the cytotoxic and bioactive effects of these biomaterials, the cell viability, metabolic activity, apoptotic properties, and DNA-synthesis activity were measured with four types of assays [Neutral Red, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, apoptotic activity, and tritiated thymidine incorporation assays] using extract and direct contact methods. Most of the biomaterials tested did not induce significant cytotoxic effects and exhibited normal metabolic function and cell growth in vitro. This normal primary human urothelial cell culture model is suitable for in vitro biocompatibility assessments and is able to provide information on the cell-biomaterial interactions and the ability of biomaterials to support bioactive cell functions.

KW - Biocompatibility

KW - Natural biomaterials

KW - Synthetic polymers

KW - Urothelial cells

UR - http://www.scopus.com/inward/record.url?scp=0035138151&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035138151&partnerID=8YFLogxK

U2 - 10.1002/1097-4636(200104)55:1<33::AID-JBM50>3.0.CO;2-7

DO - 10.1002/1097-4636(200104)55:1<33::AID-JBM50>3.0.CO;2-7

M3 - Article

C2 - 11426395

AN - SCOPUS:0035138151

VL - 55

SP - 33

EP - 39

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 1549-3296

IS - 1

ER -