Abstract
The reconstruction of urinary tissues often employs various types of biomaterials, and adequate material biocompatibility is essential for the successful reconstruction of urinary tissues. In this study we utilized a primary normal human urothelial cell culture system to evaluate the in vitro biocompatibility of a number of naturally derived biomaterials [i.e., bladder submucosa, small intestinal submucosa, collagen, and alginate] and polymeric biomaterials [i.e., poly(glycolic acid), poly(L-lactic acid), poly(lactic-co-glycolic acid), and silicone] that are either experimentally or clinically used in urinary reconstructive surgery. To determine the cytotoxic and bioactive effects of these biomaterials, the cell viability, metabolic activity, apoptotic properties, and DNA-synthesis activity were measured with four types of assays [Neutral Red, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, apoptotic activity, and tritiated thymidine incorporation assays] using extract and direct contact methods. Most of the biomaterials tested did not induce significant cytotoxic effects and exhibited normal metabolic function and cell growth in vitro. This normal primary human urothelial cell culture model is suitable for in vitro biocompatibility assessments and is able to provide information on the cell-biomaterial interactions and the ability of biomaterials to support bioactive cell functions.
| Original language | English |
|---|---|
| Pages (from-to) | 33-39 |
| Number of pages | 7 |
| Journal | Journal of Biomedical Materials Research |
| Volume | 55 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 27 2001 |
| Externally published | Yes |
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Keywords
- Biocompatibility
- Natural biomaterials
- Synthetic polymers
- Urothelial cells
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
Cite this
In vitro biocompatibility assessment of naturally derived and synthetic biomaterials using normal human urothelial cells. / Pariente, Jean Louis; Kim, Byung Soo; Atala, Anthony.
In: Journal of Biomedical Materials Research, Vol. 55, No. 1, 27.02.2001, p. 33-39.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro biocompatibility assessment of naturally derived and synthetic biomaterials using normal human urothelial cells
AU - Pariente, Jean Louis
AU - Kim, Byung Soo
AU - Atala, Anthony
PY - 2001/2/27
Y1 - 2001/2/27
N2 - The reconstruction of urinary tissues often employs various types of biomaterials, and adequate material biocompatibility is essential for the successful reconstruction of urinary tissues. In this study we utilized a primary normal human urothelial cell culture system to evaluate the in vitro biocompatibility of a number of naturally derived biomaterials [i.e., bladder submucosa, small intestinal submucosa, collagen, and alginate] and polymeric biomaterials [i.e., poly(glycolic acid), poly(L-lactic acid), poly(lactic-co-glycolic acid), and silicone] that are either experimentally or clinically used in urinary reconstructive surgery. To determine the cytotoxic and bioactive effects of these biomaterials, the cell viability, metabolic activity, apoptotic properties, and DNA-synthesis activity were measured with four types of assays [Neutral Red, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, apoptotic activity, and tritiated thymidine incorporation assays] using extract and direct contact methods. Most of the biomaterials tested did not induce significant cytotoxic effects and exhibited normal metabolic function and cell growth in vitro. This normal primary human urothelial cell culture model is suitable for in vitro biocompatibility assessments and is able to provide information on the cell-biomaterial interactions and the ability of biomaterials to support bioactive cell functions.
AB - The reconstruction of urinary tissues often employs various types of biomaterials, and adequate material biocompatibility is essential for the successful reconstruction of urinary tissues. In this study we utilized a primary normal human urothelial cell culture system to evaluate the in vitro biocompatibility of a number of naturally derived biomaterials [i.e., bladder submucosa, small intestinal submucosa, collagen, and alginate] and polymeric biomaterials [i.e., poly(glycolic acid), poly(L-lactic acid), poly(lactic-co-glycolic acid), and silicone] that are either experimentally or clinically used in urinary reconstructive surgery. To determine the cytotoxic and bioactive effects of these biomaterials, the cell viability, metabolic activity, apoptotic properties, and DNA-synthesis activity were measured with four types of assays [Neutral Red, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, apoptotic activity, and tritiated thymidine incorporation assays] using extract and direct contact methods. Most of the biomaterials tested did not induce significant cytotoxic effects and exhibited normal metabolic function and cell growth in vitro. This normal primary human urothelial cell culture model is suitable for in vitro biocompatibility assessments and is able to provide information on the cell-biomaterial interactions and the ability of biomaterials to support bioactive cell functions.
KW - Biocompatibility
KW - Natural biomaterials
KW - Synthetic polymers
KW - Urothelial cells
UR - http://www.scopus.com/inward/record.url?scp=0035138151&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035138151&partnerID=8YFLogxK
U2 - 10.1002/1097-4636(200104)55:1<33::AID-JBM50>3.0.CO;2-7
DO - 10.1002/1097-4636(200104)55:1<33::AID-JBM50>3.0.CO;2-7
M3 - Article
C2 - 11426395
AN - SCOPUS:0035138151
VL - 55
SP - 33
EP - 39
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 1
ER -