In Vitro Antimicrobial Susceptibility of Nontuberculous Mycobacteria in Iran

Parvin Heidarieh, Mehdi Mirsaeidi, Mohamad Hashemzadeh, Mohamad Mehdi Feizabadi, Saeed Zaker Bostanabad, Mostafa Ghalami Nobar, Abodolrazagh Hashemi Shahraki

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Many species of nontuberculous mycobacteria (NTM) have long been identified as important causes of human disease, the incidence of which is rising. Several reports have suggested increasing trend of both in vitro and in vivo resistance to available treatment regimes. The aim of this study was to evaluate antibiotic susceptibility of clinically relevant NTM isolates using standard microbroth dilution test. Antimicrobial susceptibility testing was performed following National Committee for Clinical Laboratory Standards methods for NTM isolates, including 85 Mycobacterium fortuitum, 39 Mycobacterium chelonae, and 30 Mycobacterium abscessus subsp. abscessus as rapidly growing mycobacteria and 48 Mycobacterium simiae and 40 Mycobacterium kansasii as slowly growing mycobacteria. All isolates were recovered from various types of clinical samples and identified by multilocus sequence analysis. Trimethoprim-sulfamethoxazole (TMP-SMZ), amikacin, tobramycin, clarithromycin, moxifloxacin, linezolid, and imipenem showed better activity against M. fortuitum rather than meropenem, ciprofloxacin, cefoxitin, and doxycycline. Amikacin was active against 93% of M. abscessus subsp. abscessus. Linezolid, clarithromycin, cefoxitin, ciprofloxacin, imipenem, moxifloxacin, tobramycin, TMP-SMZ, doxycycline, and meropenem showed some activities on M. abscessus subsp. abscessus as well. The majority of M. abscessus subsp. abscessus and M. chelonae strains were multidrug resistant. Among the 40 isolates of M. kansasii, all were susceptible to ethambutol, isoniazid, clarithromycin, moxifloxacin, and linezolid. These isolates were also resistant to doxycycline and 50% were resistant to rifampicin and ciprofloxacin. M. simiae was resistant to clarithromycin, doxycycline, isoniazid, and TMP-SMZ, and the majority of isolates showed high levels of resistance to linezolid, ethambutol, ciprofloxacin, streptomycin, and rifampicin. The majority of M. simiae isolates were multidrug resistant. Our data confirm the need for performing of standard susceptibility testing of any clinically important NTM isolate.

Original languageEnglish (US)
Pages (from-to)172-178
Number of pages7
JournalMicrobial Drug Resistance
Volume22
Issue number2
DOIs
StatePublished - Mar 1 2016

Fingerprint

Linezolid
Nontuberculous Mycobacteria
Clarithromycin
Doxycycline
meropenem
Mycobacterium
Ciprofloxacin
Iran
Sulfamethoxazole Drug Combination Trimethoprim
Mycobacterium chelonae
Mycobacterium kansasii
Mycobacterium fortuitum
Cefoxitin
Ethambutol
Tobramycin
Amikacin
Imipenem
Isoniazid
Rifampin
Multilocus Sequence Typing

ASJC Scopus subject areas

  • Microbiology (medical)
  • Pharmacology
  • Immunology
  • Microbiology

Cite this

Heidarieh, P., Mirsaeidi, M., Hashemzadeh, M., Feizabadi, M. M., Bostanabad, S. Z., Nobar, M. G., & Shahraki, A. H. (2016). In Vitro Antimicrobial Susceptibility of Nontuberculous Mycobacteria in Iran. Microbial Drug Resistance, 22(2), 172-178. https://doi.org/10.1089/mdr.2015.0134

In Vitro Antimicrobial Susceptibility of Nontuberculous Mycobacteria in Iran. / Heidarieh, Parvin; Mirsaeidi, Mehdi; Hashemzadeh, Mohamad; Feizabadi, Mohamad Mehdi; Bostanabad, Saeed Zaker; Nobar, Mostafa Ghalami; Shahraki, Abodolrazagh Hashemi.

In: Microbial Drug Resistance, Vol. 22, No. 2, 01.03.2016, p. 172-178.

Research output: Contribution to journalArticle

Heidarieh, P, Mirsaeidi, M, Hashemzadeh, M, Feizabadi, MM, Bostanabad, SZ, Nobar, MG & Shahraki, AH 2016, 'In Vitro Antimicrobial Susceptibility of Nontuberculous Mycobacteria in Iran', Microbial Drug Resistance, vol. 22, no. 2, pp. 172-178. https://doi.org/10.1089/mdr.2015.0134
Heidarieh P, Mirsaeidi M, Hashemzadeh M, Feizabadi MM, Bostanabad SZ, Nobar MG et al. In Vitro Antimicrobial Susceptibility of Nontuberculous Mycobacteria in Iran. Microbial Drug Resistance. 2016 Mar 1;22(2):172-178. https://doi.org/10.1089/mdr.2015.0134
Heidarieh, Parvin ; Mirsaeidi, Mehdi ; Hashemzadeh, Mohamad ; Feizabadi, Mohamad Mehdi ; Bostanabad, Saeed Zaker ; Nobar, Mostafa Ghalami ; Shahraki, Abodolrazagh Hashemi. / In Vitro Antimicrobial Susceptibility of Nontuberculous Mycobacteria in Iran. In: Microbial Drug Resistance. 2016 ; Vol. 22, No. 2. pp. 172-178.
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