In vitro and in vivo evaluation of the comparative thrombogenicity of cellulose acetate hemodialyzers with radiolabeled platelets

M. K. Dewanjee, M. Kapadvanjwala, C. F. Cavagnaro, G. K. Panoutsopoulos, C. Y. Suguihara, R. Elson, Shabbir Ezuddin, Aldo N Serafini, Gaston E Zilleruelo, George N Sfakianakis

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Platelet consumption and platelet kinetics during hemodialysis were quantified in Yorkshire pigs with In-111 labeled platelets. Six anesthetized pigs (20-25 kg) were hemodialyzed at 150 ml/min for 3 hr. All pigs were injected with autologous In-111 labeled platelets (300-420 μCi) 24 hr before dialysis and were systemically heparinized (ACT > 400 sec) before cannulation. Hemodialysis was instituted with a Drake-Willock hemodialysis machine and a hollow fiber dialyzer (Cobe4, 0.6 m2). In vitro sham dialysis was carried out at 150 ml/min for 3 hr with six more dialyzers in a flow- loop with the blood reservoir maintained at 37°C. In vitro thrombogenicity over-estimates (10-fold) in vivo values. In both systems, platelet deposition on dialyzers reached a steady state, suggesting a constant rate of thrombus formation and embolization in the hollow fiber system. The relative thrombus distribution after 3 hr of dialysis was similar in both systems, with adherent thrombi in the entry and exit ports and highest numbers in the midsection of the hemodialyzer. Biodistribution after 3 hr of dialysis indicated that thrombosis of the hemodialyzer and arterial and venous traps as well as embolization reduced the platelet pool in the blood and increased platelet emboli in lung, brain, kidneys, and skeletal muscle, as measured by the In-111 labeled platelets.

Original languageEnglish
Pages (from-to)49-55
Number of pages7
JournalASAIO Journal
Volume40
Issue number1
StatePublished - Jan 1 1994

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Hemodialyzers
Artificial Kidneys
Platelets
Cellulose
Blood Platelets
Dialysis
Thrombosis
Renal Dialysis
Swine
Blood
In Vitro Techniques
acetylcellulose
Fibers
Embolism
Catheterization
Muscle
Brain
Skeletal Muscle

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering

Cite this

Dewanjee, M. K., Kapadvanjwala, M., Cavagnaro, C. F., Panoutsopoulos, G. K., Suguihara, C. Y., Elson, R., ... Sfakianakis, G. N. (1994). In vitro and in vivo evaluation of the comparative thrombogenicity of cellulose acetate hemodialyzers with radiolabeled platelets. ASAIO Journal, 40(1), 49-55.

In vitro and in vivo evaluation of the comparative thrombogenicity of cellulose acetate hemodialyzers with radiolabeled platelets. / Dewanjee, M. K.; Kapadvanjwala, M.; Cavagnaro, C. F.; Panoutsopoulos, G. K.; Suguihara, C. Y.; Elson, R.; Ezuddin, Shabbir; Serafini, Aldo N; Zilleruelo, Gaston E; Sfakianakis, George N.

In: ASAIO Journal, Vol. 40, No. 1, 01.01.1994, p. 49-55.

Research output: Contribution to journalArticle

Dewanjee, MK, Kapadvanjwala, M, Cavagnaro, CF, Panoutsopoulos, GK, Suguihara, CY, Elson, R, Ezuddin, S, Serafini, AN, Zilleruelo, GE & Sfakianakis, GN 1994, 'In vitro and in vivo evaluation of the comparative thrombogenicity of cellulose acetate hemodialyzers with radiolabeled platelets', ASAIO Journal, vol. 40, no. 1, pp. 49-55.
Dewanjee MK, Kapadvanjwala M, Cavagnaro CF, Panoutsopoulos GK, Suguihara CY, Elson R et al. In vitro and in vivo evaluation of the comparative thrombogenicity of cellulose acetate hemodialyzers with radiolabeled platelets. ASAIO Journal. 1994 Jan 1;40(1):49-55.
Dewanjee, M. K. ; Kapadvanjwala, M. ; Cavagnaro, C. F. ; Panoutsopoulos, G. K. ; Suguihara, C. Y. ; Elson, R. ; Ezuddin, Shabbir ; Serafini, Aldo N ; Zilleruelo, Gaston E ; Sfakianakis, George N. / In vitro and in vivo evaluation of the comparative thrombogenicity of cellulose acetate hemodialyzers with radiolabeled platelets. In: ASAIO Journal. 1994 ; Vol. 40, No. 1. pp. 49-55.
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