In situ regeneration of skeletal muscle tissue through host cell recruitment

Young M in Ju, Anthony Atala, James J. Yoo, Sang J in Lee

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Standard reconstructive procedures for restoring normal function after skeletal muscle defects involve the use of existing host tissues such as muscular flaps. In many instances, this approach is not feasible and delays the rehabilitation process and restoration of tissue function. Currently, cell-based tissue engineering strategies have been used for reconstruction; however, donor tissue biopsy and ex vivo cell manipulation are required prior to implantation. The present study aimed to overcome these limitations by demonstrating mobilization of muscle cells into a target-specific site for in situ muscle regeneration. First, we investigated whether host muscle cells could be mobilized into an implanted scaffold. Poly(l-lactic acid) (PLLA) scaffolds were implanted in the tibialis anterior (TA) muscle of rats, and the retrieved scaffolds were characterized by examining host cell infiltration in the scaffolds. The host cell infiltrates, including Pax7+ cells, gradually increased with time. Second, we demonstrated that host muscle cells could be enriched by a myogenic factor released from the scaffolds. Gelatin-based scaffolds containing a myogenic factor were implanted in the TA muscle of rats, and the Pax7+ cell infiltration and newly formed muscle fibers were examined. By the second week after implantation, the Pax7+ cell infiltrates and muscle formation were significantly accelerated within the scaffolds containing insulin-like growth factor 1 (IGF-1). Our data suggest an ability of host stem cells to be recruited into the scaffolds with the capability of differentiating to muscle cells. In addition, the myogenic factor effectively promoted host cell recruitment, which resulted in accelerating muscle regeneration in situ.

Original languageEnglish (US)
Pages (from-to)4332-4339
Number of pages8
JournalActa Biomaterialia
Volume10
Issue number10
DOIs
StatePublished - Oct 1 2014
Externally publishedYes

Fingerprint

Muscle
Regeneration
Skeletal Muscle
Scaffolds
Tissue
Muscle Cells
Muscles
Cells
Infiltration
Rats
Somatomedins
Gelatin
Tissue Engineering
Flaps
Stem Cells
Rehabilitation
Biopsy
Tissue Donors
Insulin
Lactic acid

Keywords

  • Biomaterials
  • Reconstruction
  • Skeletal muscle
  • Stem cells
  • Tissue engineering

ASJC Scopus subject areas

  • Medicine(all)

Cite this

In situ regeneration of skeletal muscle tissue through host cell recruitment. / Ju, Young M in; Atala, Anthony; Yoo, James J.; Lee, Sang J in.

In: Acta Biomaterialia, Vol. 10, No. 10, 01.10.2014, p. 4332-4339.

Research output: Contribution to journalArticle

Ju, Young M in ; Atala, Anthony ; Yoo, James J. ; Lee, Sang J in. / In situ regeneration of skeletal muscle tissue through host cell recruitment. In: Acta Biomaterialia. 2014 ; Vol. 10, No. 10. pp. 4332-4339.
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