In situ enrichment of Ocean crust microbes on igneous minerals and glasses using an osmotic flow-through device

Amy Smith, Radu Popa, Martin Fisk, Mark Nielsen, C. Geoffrey Wheat, Hans W. Jannasch, Andrew T. Fisher, Keir Becker, Stefan M. Sievert, Gilberto Flores

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The Integrated Ocean Drilling Program (IODP) Hole 1301A on the eastern flank of Juan de Fuca Ridge was used in the first long-term deployment of microbial enrichment flow cells using osmotically driven pumps in a subseafloor borehole. Three novel osmotically driven colonization systems with unidirectional flow were deployed in the borehole and incubated for 4 years to determine the microbial colonization preferences for 12 minerals and glasses present in igneous rocks. Following recovery of the colonization systems, we measured cell density on the minerals and glasses by fluorescent staining and direct counting and found some significant differences between mineral samples. We also determined the abundance of mesophilic and thermophilic culturable organotrophs grown on marine R2A medium and identified isolates by partial 16S or 18S rDNA sequencing. We found that nine distinct phylotypes of culturable mesophilic oligotrophs were present on the minerals and glasses and that eight of the nine can reduce nitrate and oxidize iron. Fe(II)-rich olivine minerals had the highest density of total countable cells and culturable organotrophic mesophiles, as well as the only culturable organotrophic thermophiles. These results suggest that olivine (a common igneous mineral) in seawater-recharged ocean crust is capable of supporting microbial communities, that iron oxidation and nitrate reduction may be important physiological characteristics of ocean crust microbes, and that heterogeneously distributed minerals in marine igneous rocks likely influence the distribution of microbial communities in the ocean crust.

Original languageEnglish (US)
Article numberQ06007
JournalGeochemistry, Geophysics, Geosystems
Volume12
Issue number6
DOIs
StatePublished - 2011

Fingerprint

microorganisms
Minerals
crusts
oceans
glass
minerals
crust
Glass
ocean
mineral
Igneous rocks
colonization
igneous rocks
boreholes
Boreholes
olivine
Nitrates
igneous rock
nitrates
microbial community

Keywords

  • basalt
  • iron oxidizers
  • Juan de Fuca
  • nitrate reducers
  • olivine
  • subseafloor

ASJC Scopus subject areas

  • Geochemistry and Petrology
  • Geophysics

Cite this

In situ enrichment of Ocean crust microbes on igneous minerals and glasses using an osmotic flow-through device. / Smith, Amy; Popa, Radu; Fisk, Martin; Nielsen, Mark; Wheat, C. Geoffrey; Jannasch, Hans W.; Fisher, Andrew T.; Becker, Keir; Sievert, Stefan M.; Flores, Gilberto.

In: Geochemistry, Geophysics, Geosystems, Vol. 12, No. 6, Q06007, 2011.

Research output: Contribution to journalArticle

Smith, A, Popa, R, Fisk, M, Nielsen, M, Wheat, CG, Jannasch, HW, Fisher, AT, Becker, K, Sievert, SM & Flores, G 2011, 'In situ enrichment of Ocean crust microbes on igneous minerals and glasses using an osmotic flow-through device', Geochemistry, Geophysics, Geosystems, vol. 12, no. 6, Q06007. https://doi.org/10.1029/2010GC003424
Smith, Amy ; Popa, Radu ; Fisk, Martin ; Nielsen, Mark ; Wheat, C. Geoffrey ; Jannasch, Hans W. ; Fisher, Andrew T. ; Becker, Keir ; Sievert, Stefan M. ; Flores, Gilberto. / In situ enrichment of Ocean crust microbes on igneous minerals and glasses using an osmotic flow-through device. In: Geochemistry, Geophysics, Geosystems. 2011 ; Vol. 12, No. 6.
@article{89aa34240eed4f14a27a7fbc88db9594,
title = "In situ enrichment of Ocean crust microbes on igneous minerals and glasses using an osmotic flow-through device",
abstract = "The Integrated Ocean Drilling Program (IODP) Hole 1301A on the eastern flank of Juan de Fuca Ridge was used in the first long-term deployment of microbial enrichment flow cells using osmotically driven pumps in a subseafloor borehole. Three novel osmotically driven colonization systems with unidirectional flow were deployed in the borehole and incubated for 4 years to determine the microbial colonization preferences for 12 minerals and glasses present in igneous rocks. Following recovery of the colonization systems, we measured cell density on the minerals and glasses by fluorescent staining and direct counting and found some significant differences between mineral samples. We also determined the abundance of mesophilic and thermophilic culturable organotrophs grown on marine R2A medium and identified isolates by partial 16S or 18S rDNA sequencing. We found that nine distinct phylotypes of culturable mesophilic oligotrophs were present on the minerals and glasses and that eight of the nine can reduce nitrate and oxidize iron. Fe(II)-rich olivine minerals had the highest density of total countable cells and culturable organotrophic mesophiles, as well as the only culturable organotrophic thermophiles. These results suggest that olivine (a common igneous mineral) in seawater-recharged ocean crust is capable of supporting microbial communities, that iron oxidation and nitrate reduction may be important physiological characteristics of ocean crust microbes, and that heterogeneously distributed minerals in marine igneous rocks likely influence the distribution of microbial communities in the ocean crust.",
keywords = "basalt, iron oxidizers, Juan de Fuca, nitrate reducers, olivine, subseafloor",
author = "Amy Smith and Radu Popa and Martin Fisk and Mark Nielsen and Wheat, {C. Geoffrey} and Jannasch, {Hans W.} and Fisher, {Andrew T.} and Keir Becker and Sievert, {Stefan M.} and Gilberto Flores",
year = "2011",
doi = "10.1029/2010GC003424",
language = "English (US)",
volume = "12",
journal = "Geochemistry, Geophysics, Geosystems",
issn = "1525-2027",
publisher = "American Geophysical Union",
number = "6",

}

TY - JOUR

T1 - In situ enrichment of Ocean crust microbes on igneous minerals and glasses using an osmotic flow-through device

AU - Smith, Amy

AU - Popa, Radu

AU - Fisk, Martin

AU - Nielsen, Mark

AU - Wheat, C. Geoffrey

AU - Jannasch, Hans W.

AU - Fisher, Andrew T.

AU - Becker, Keir

AU - Sievert, Stefan M.

AU - Flores, Gilberto

PY - 2011

Y1 - 2011

N2 - The Integrated Ocean Drilling Program (IODP) Hole 1301A on the eastern flank of Juan de Fuca Ridge was used in the first long-term deployment of microbial enrichment flow cells using osmotically driven pumps in a subseafloor borehole. Three novel osmotically driven colonization systems with unidirectional flow were deployed in the borehole and incubated for 4 years to determine the microbial colonization preferences for 12 minerals and glasses present in igneous rocks. Following recovery of the colonization systems, we measured cell density on the minerals and glasses by fluorescent staining and direct counting and found some significant differences between mineral samples. We also determined the abundance of mesophilic and thermophilic culturable organotrophs grown on marine R2A medium and identified isolates by partial 16S or 18S rDNA sequencing. We found that nine distinct phylotypes of culturable mesophilic oligotrophs were present on the minerals and glasses and that eight of the nine can reduce nitrate and oxidize iron. Fe(II)-rich olivine minerals had the highest density of total countable cells and culturable organotrophic mesophiles, as well as the only culturable organotrophic thermophiles. These results suggest that olivine (a common igneous mineral) in seawater-recharged ocean crust is capable of supporting microbial communities, that iron oxidation and nitrate reduction may be important physiological characteristics of ocean crust microbes, and that heterogeneously distributed minerals in marine igneous rocks likely influence the distribution of microbial communities in the ocean crust.

AB - The Integrated Ocean Drilling Program (IODP) Hole 1301A on the eastern flank of Juan de Fuca Ridge was used in the first long-term deployment of microbial enrichment flow cells using osmotically driven pumps in a subseafloor borehole. Three novel osmotically driven colonization systems with unidirectional flow were deployed in the borehole and incubated for 4 years to determine the microbial colonization preferences for 12 minerals and glasses present in igneous rocks. Following recovery of the colonization systems, we measured cell density on the minerals and glasses by fluorescent staining and direct counting and found some significant differences between mineral samples. We also determined the abundance of mesophilic and thermophilic culturable organotrophs grown on marine R2A medium and identified isolates by partial 16S or 18S rDNA sequencing. We found that nine distinct phylotypes of culturable mesophilic oligotrophs were present on the minerals and glasses and that eight of the nine can reduce nitrate and oxidize iron. Fe(II)-rich olivine minerals had the highest density of total countable cells and culturable organotrophic mesophiles, as well as the only culturable organotrophic thermophiles. These results suggest that olivine (a common igneous mineral) in seawater-recharged ocean crust is capable of supporting microbial communities, that iron oxidation and nitrate reduction may be important physiological characteristics of ocean crust microbes, and that heterogeneously distributed minerals in marine igneous rocks likely influence the distribution of microbial communities in the ocean crust.

KW - basalt

KW - iron oxidizers

KW - Juan de Fuca

KW - nitrate reducers

KW - olivine

KW - subseafloor

UR - http://www.scopus.com/inward/record.url?scp=79959586513&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959586513&partnerID=8YFLogxK

U2 - 10.1029/2010GC003424

DO - 10.1029/2010GC003424

M3 - Article

AN - SCOPUS:79959586513

VL - 12

JO - Geochemistry, Geophysics, Geosystems

JF - Geochemistry, Geophysics, Geosystems

SN - 1525-2027

IS - 6

M1 - Q06007

ER -