In situ cell manipulation through enzymatic hydrogel photopatterning

Katarzyna A. Mosiewicz, Laura Kolb, André J. Van Der Vlies, Mikaël M. Martino, Philipp S. Lienemann, Jeffrey A. Hubbell, Martin Ehrbar, Matthias P. Lutolf

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

The physicochemical properties of hydrogels can be manipulated in both space and time through the controlled application of a light beam. However, methods for hydrogel photopatterning either fail to maintain the bioactivity of fragile proteins and are thus limited to short peptides, or have been used in hydrogels that often do not support three-dimensional (3D) cell growth. Here, we show that the 3D invasion of primary human mesenchymal stem cells can be spatiotemporally controlled by micropatterning the hydrogel with desired extracellular matrix (ECM) proteins and growth factors. A peptide substrate of activated transglutaminase factor XIII (FXIIIa) - a key ECM crosslinking enzyme - is rendered photosensitive by masking its active site with a photolabile cage group. Covalent incorporation of the caged FXIIIa substrate into poly(ethylene glycol) hydrogels and subsequent laser-scanning lithography affords highly localized biomolecule tethering. This approach for the 3D manipulation of cells within gels should open up avenues for the study and manipulation of cell signalling.

Original languageEnglish
Pages (from-to)1072-1078
Number of pages7
JournalNature Materials
Volume12
Issue number11
DOIs
StatePublished - Nov 1 2013
Externally publishedYes

Fingerprint

Hydrogels
Hydrogel
peptides
manipulators
tethering
proteins
stem cells
crosslinking
matrices
masking
cells
light beams
Cell signaling
Factor XIII
enzymes
glycols
Peptides
Transglutaminases
ethylene
lithography

ASJC Scopus subject areas

  • Mechanical Engineering
  • Mechanics of Materials
  • Condensed Matter Physics
  • Materials Science(all)
  • Chemistry(all)

Cite this

Mosiewicz, K. A., Kolb, L., Van Der Vlies, A. J., Martino, M. M., Lienemann, P. S., Hubbell, J. A., ... Lutolf, M. P. (2013). In situ cell manipulation through enzymatic hydrogel photopatterning. Nature Materials, 12(11), 1072-1078. https://doi.org/10.1038/nmat3766

In situ cell manipulation through enzymatic hydrogel photopatterning. / Mosiewicz, Katarzyna A.; Kolb, Laura; Van Der Vlies, André J.; Martino, Mikaël M.; Lienemann, Philipp S.; Hubbell, Jeffrey A.; Ehrbar, Martin; Lutolf, Matthias P.

In: Nature Materials, Vol. 12, No. 11, 01.11.2013, p. 1072-1078.

Research output: Contribution to journalArticle

Mosiewicz, KA, Kolb, L, Van Der Vlies, AJ, Martino, MM, Lienemann, PS, Hubbell, JA, Ehrbar, M & Lutolf, MP 2013, 'In situ cell manipulation through enzymatic hydrogel photopatterning', Nature Materials, vol. 12, no. 11, pp. 1072-1078. https://doi.org/10.1038/nmat3766
Mosiewicz KA, Kolb L, Van Der Vlies AJ, Martino MM, Lienemann PS, Hubbell JA et al. In situ cell manipulation through enzymatic hydrogel photopatterning. Nature Materials. 2013 Nov 1;12(11):1072-1078. https://doi.org/10.1038/nmat3766
Mosiewicz, Katarzyna A. ; Kolb, Laura ; Van Der Vlies, André J. ; Martino, Mikaël M. ; Lienemann, Philipp S. ; Hubbell, Jeffrey A. ; Ehrbar, Martin ; Lutolf, Matthias P. / In situ cell manipulation through enzymatic hydrogel photopatterning. In: Nature Materials. 2013 ; Vol. 12, No. 11. pp. 1072-1078.
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