In silico search for the expressed sequences in the region q14.3 of human chromosome 13

T. V. Tyazhelova, D. V. Ivanov, S. A. Nazarenko, A. V. Baranova, N. K. Yankovsky

Research output: Contribution to journalArticle

Abstract

Using computer-aided genomic methods, a complete map of the expressed sequence tags (EST) located in the human genome region 13q14.3 between the STS markers, D13S810 and D13S1469, was constructed. A total of 62 EST clusters were formed, of which 12 clusters corresponded to the already known human genes, 4 clusters represented pseudogenes, and 10 clusters were new human genes. The use of the method of reverse transcription in combination with polymerase chain reaction (RT-PCR) provided experimental confirmation of the existence of mRNA transcripts for the novel human genes revealed in silico.

Original languageEnglish (US)
Pages (from-to)422-426
Number of pages5
JournalGenetika
Volume40
Issue number3
StatePublished - Dec 1 2004
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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    Tyazhelova, T. V., Ivanov, D. V., Nazarenko, S. A., Baranova, A. V., & Yankovsky, N. K. (2004). In silico search for the expressed sequences in the region q14.3 of human chromosome 13. Genetika, 40(3), 422-426.