Improving lateral resolution and image quality of optical coherence tomography by the multiframe superresolution technique for 3D tissue imaging

Kai Shen, Hui Lu, Sarfaraz Baig, Michael Renxun Wang

Research output: Contribution to journalArticle

5 Scopus citations


The multi-frame superresolution technique is introduced to significantly improve the lateral resolution and image quality of spectral domain optical coherence tomography (SD-OCT). Using several sets of low resolution C-scan 3D images with lateral sub-spotspacing shifts on different sets, the multi-frame superresolution processing of these sets at each depth layer reconstructs a higher resolution and quality lateral image. Layer by layer processing yields an overall high lateral resolution and quality 3D image. In theory, the superresolution processing including deconvolution can solve the diffraction limit, lateral scan density and background noise problems together. In experiment, the improved lateral resolution by ~3 times reaching 7.81 μm and 2.19 μm using sample arm optics of 0.015 and 0.05 numerical aperture respectively as well as doubling the image quality has been confirmed by imaging a known resolution test target. Improved lateral resolution on in vitro skin C-scan images has been demonstrated. For in vivo 3D SD-OCT imaging of human skin, fingerprint and retina layer, we used the multi-modal volume registration method to effectively estimate the lateral image shifts among different C-scans due to random minor unintended live body motion. Further processing of these images generated high lateral resolution 3D images as well as high quality B-scan images of these in vivo tissues.

Original languageEnglish (US)
Article number#297772
Pages (from-to)4887-4918
Number of pages32
JournalBiomedical Optics Express
Issue number11
StatePublished - Nov 1 2017


ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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