Improved genetic stability of recombinant yellow fever 17d virus expressing a lentiviral gag gene fragment

Marlon G.Veloso de Santana, Patrícia C.C. Neves, Juliana Ribeiro dos Santos, Noemia S. Lima, Alexandre A.C.dos Santos, David I. Watkins, Ricardo Galler, Myrna C. Bonaldo

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We have previously designed a method to construct viable recombinant Yellow Fever (YF) 17D viruses expressing heterologous polypeptides including part of the Simian Immunodeficiency Virus (SIV) Gag protein. However, the expressed region, encompassing amino acid residues from 45 to 269, was genetically unstable. In this study, we improved the genetic stability of this recombinant YF 17D virus by introducing mutations in the IRES element localized at the 5' end of the SIV gag gene. The new stable recombinant virus elicited adaptive immune responses similar to those induced by the original recombinant virus. It is, therefore, possible to increase recombinant stability by removing functional motifs from the insert that may have deleterious effects on recombinant YF viral fitness.

Original languageEnglish (US)
Pages (from-to)202-211
Number of pages10
JournalVirology
Volume452-453
DOIs
StatePublished - Mar 1 2014

Keywords

  • Genetic stability
  • Recombinant virus
  • SIV Gag
  • SIV gag IRES
  • Viral vector
  • Yellow Fever 17D virus

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Improved genetic stability of recombinant yellow fever 17d virus expressing a lentiviral gag gene fragment'. Together they form a unique fingerprint.

  • Cite this

    Santana, M. G. V. D., Neves, P. C. C., Santos, J. R. D., Lima, N. S., Santos, A. A. C. D., Watkins, D. I., Galler, R., & Bonaldo, M. C. (2014). Improved genetic stability of recombinant yellow fever 17d virus expressing a lentiviral gag gene fragment. Virology, 452-453, 202-211. https://doi.org/10.1016/j.virol.2014.01.017