@article{e8c651d866e64b388e16f21fc94155fe,
title = "Improved genetic stability of recombinant yellow fever 17d virus expressing a lentiviral gag gene fragment",
abstract = "We have previously designed a method to construct viable recombinant Yellow Fever (YF) 17D viruses expressing heterologous polypeptides including part of the Simian Immunodeficiency Virus (SIV) Gag protein. However, the expressed region, encompassing amino acid residues from 45 to 269, was genetically unstable. In this study, we improved the genetic stability of this recombinant YF 17D virus by introducing mutations in the IRES element localized at the 5' end of the SIV gag gene. The new stable recombinant virus elicited adaptive immune responses similar to those induced by the original recombinant virus. It is, therefore, possible to increase recombinant stability by removing functional motifs from the insert that may have deleterious effects on recombinant YF viral fitness.",
keywords = "Genetic stability, Recombinant virus, SIV Gag, SIV gag IRES, Viral vector, Yellow Fever 17D virus",
author = "Santana, {Marlon G.Veloso de} and Neves, {Patr{\'i}cia C.C.} and Santos, {Juliana Ribeiro dos} and Lima, {Noemia S.} and Santos, {Alexandre A.C.dos} and Watkins, {David I.} and Ricardo Galler and Bonaldo, {Myrna C.}",
note = "Funding Information: The authors are in debt to PDTIS-FIOCRUZ for supporting the sequencing, ELISpot and confocal microscopy studies through the Genomics, ELISpot and Confocal Microscopy Facilities. We are also grateful to Heloisa Diniz of Servi{\c c}o de Produ{\c c}{\~a}o e Tratamento de Imagem – IOC/FIOCRUZ and Marcia de Souza Santana for technical support with the figures presented in this work and to Richard Rudersdorf for technical assistance. This work was supported by Grants from Funda{\c c}{\~a}o de Amparo {\`a} Pesquisa do Estado de Rio de Janeiro (Faperj) , The National Institute for Vaccine Science and Technology (INCTV, MCT/CNPq) , Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq) , Fiocruz and National Institutes of Health (NIH) HIVRAD Grant 1P01AI094420-01A1 . ",
year = "2014",
month = mar,
doi = "10.1016/j.virol.2014.01.017",
language = "English (US)",
volume = "452-453",
pages = "202--211",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
}