Improved genetic stability of recombinant yellow fever 17d virus expressing a lentiviral gag gene fragment

Marlon G.Veloso de Santana; Patrícia C.C. Neves; Juliana Ribeiro dos Santos; Noemia S. Lima; Alexandre A.C.dos Santos; David I. Watkins; Ricardo Galler; Myrna C. Bonaldo

doi:10.1016/j.virol.2014.01.017

Improved genetic stability of recombinant yellow fever 17d virus expressing a lentiviral gag gene fragment

Marlon G.Veloso de Santana, Patrícia C.C. Neves, Juliana Ribeiro dos Santos, Noemia S. Lima, Alexandre A.C.dos Santos, David I. Watkins, Ricardo Galler, Myrna C. Bonaldo

Pathology

Research output: Contribution to journal › Article

6 Scopus citations

Abstract

We have previously designed a method to construct viable recombinant Yellow Fever (YF) 17D viruses expressing heterologous polypeptides including part of the Simian Immunodeficiency Virus (SIV) Gag protein. However, the expressed region, encompassing amino acid residues from 45 to 269, was genetically unstable. In this study, we improved the genetic stability of this recombinant YF 17D virus by introducing mutations in the IRES element localized at the 5' end of the SIV gag gene. The new stable recombinant virus elicited adaptive immune responses similar to those induced by the original recombinant virus. It is, therefore, possible to increase recombinant stability by removing functional motifs from the insert that may have deleterious effects on recombinant YF viral fitness.

Original language	English (US)
Pages (from-to)	202-211
Number of pages	10
Journal	Virology
Volume	452-453
DOIs	https://doi.org/10.1016/j.virol.2014.01.017
State	Published - Mar 1 2014

Keywords

Genetic stability
Recombinant virus
SIV Gag
SIV gag IRES
Viral vector
Yellow Fever 17D virus

ASJC Scopus subject areas

Virology

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Santana, M. G. V. D., Neves, P. C. C., Santos, J. R. D., Lima, N. S., Santos, A. A. C. D., Watkins, D. I., Galler, R., & Bonaldo, M. C. (2014). Improved genetic stability of recombinant yellow fever 17d virus expressing a lentiviral gag gene fragment. Virology, 452-453, 202-211. https://doi.org/10.1016/j.virol.2014.01.017

Improved genetic stability of recombinant yellow fever 17d virus expressing a lentiviral gag gene fragment. / Santana, Marlon G.Veloso de; Neves, Patrícia C.C.; Santos, Juliana Ribeiro dos; Lima, Noemia S.; Santos, Alexandre A.C.dos; Watkins, David I.; Galler, Ricardo; Bonaldo, Myrna C.

In: Virology, Vol. 452-453, 01.03.2014, p. 202-211.

Research output: Contribution to journal › Article

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abstract = "We have previously designed a method to construct viable recombinant Yellow Fever (YF) 17D viruses expressing heterologous polypeptides including part of the Simian Immunodeficiency Virus (SIV) Gag protein. However, the expressed region, encompassing amino acid residues from 45 to 269, was genetically unstable. In this study, we improved the genetic stability of this recombinant YF 17D virus by introducing mutations in the IRES element localized at the 5' end of the SIV gag gene. The new stable recombinant virus elicited adaptive immune responses similar to those induced by the original recombinant virus. It is, therefore, possible to increase recombinant stability by removing functional motifs from the insert that may have deleterious effects on recombinant YF viral fitness.",

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AU - Santos, Alexandre A.C.dos

AU - Watkins, David I.

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