Improved Detection of Antibodies to Hepatitis C Virus in Dialysis Patients Using a Second-Generation Enzyme Immunoassay

Maria de Medina, Carmen Ortiz, Christina Krenc, Jean Leete, David Vallari, Mary Hill, Silvia LaRue, Margarita Jimenez, William Anderson, Eugene Schiff, Guido Perez

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

We tested serum samples from 99 patients undergoing maintenance hemodialysis for hepatitis C virus (HCV) antibodies using a first-generation, licensed anti-HCV enzyme immunoassay (EIA) and a second-generation anti-HCV EIA that detect three gene products c100-3, NS3, and core. Specimens that were repeatedly reactive by either or both screening assays were further evaluated by testing with supplemental EIAs and a dot blot immunoassay. There was 87.9% agreement between the licensed HCV EIA and the HCV EIA second generation. HCV EIA Second Generation detected 10 more positive specimens than HCV EIA, for an increase in detection from 33.3% (33/99) to 43.4% (43/99). We conclude that HCV EIA Second Generation improves detection of HCV infection in hemodialysis patients.

Original languageEnglish (US)
Pages (from-to)589-591
Number of pages3
JournalAmerican Journal of Kidney Diseases
Volume20
Issue number6
DOIs
StatePublished - Jan 1 1992

Keywords

  • Hepatitis C
  • enzyme immunoassay
  • hemodialysis

ASJC Scopus subject areas

  • Nephrology

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    de Medina, M., Ortiz, C., Krenc, C., Leete, J., Vallari, D., Hill, M., LaRue, S., Jimenez, M., Anderson, W., Schiff, E., & Perez, G. (1992). Improved Detection of Antibodies to Hepatitis C Virus in Dialysis Patients Using a Second-Generation Enzyme Immunoassay. American Journal of Kidney Diseases, 20(6), 589-591. https://doi.org/10.1016/S0272-6386(12)70224-X