Improved coverage and accuracy with strand-conserving sequence enrichment

Toumy Guettouche; Stephan Zuchner

doi:10.1186/gm450

Improved coverage and accuracy with strand-conserving sequence enrichment

Toumy Guettouche, Stephan Zuchner

Hussman Institute for Human Genomics

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Targeted next-generation sequencing is becoming a common tool in the molecular diagnostic laboratory. However, currently available methods to enrich for regions of interest in the DNA sequence suffer from drawbacks such as high cost, complex protocols, lack of clinical-level accuracy and uneven target coverage. A target-enrichment approach using complementary long padlock probes described in a recent article significantly improves on previous methods in most of these areas. See related Research: http://genomemedicine.com/content/5/5/50.

Original language	English (US)
Article number	46
Journal	Genome Medicine
Volume	5
Issue number	5
DOIs	https://doi.org/10.1186/gm450
State	Published - May 29 2013

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Genetics
Genetics(clinical)

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Improved coverage and accuracy with strand-conserving sequence enrichment

Abstract

ASJC Scopus subject areas

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