Abstract
Targeted next-generation sequencing is becoming a common tool in the molecular diagnostic laboratory. However, currently available methods to enrich for regions of interest in the DNA sequence suffer from drawbacks such as high cost, complex protocols, lack of clinical-level accuracy and uneven target coverage. A target-enrichment approach using complementary long padlock probes described in a recent article significantly improves on previous methods in most of these areas. See related Research: http://genomemedicine.com/content/5/5/50.
| Original language | English |
|---|---|
| Article number | 46 |
| Journal | Genome Medicine |
| Volume | 5 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 29 2013 |
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ASJC Scopus subject areas
- Genetics(clinical)
- Genetics
- Molecular Biology
- Molecular Medicine
Cite this
Improved coverage and accuracy with strand-conserving sequence enrichment. / Guettouche, Toumy; Zuchner, Stephan L.
In: Genome Medicine, Vol. 5, No. 5, 46, 29.05.2013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Improved coverage and accuracy with strand-conserving sequence enrichment
AU - Guettouche, Toumy
AU - Zuchner, Stephan L
PY - 2013/5/29
Y1 - 2013/5/29
N2 - Targeted next-generation sequencing is becoming a common tool in the molecular diagnostic laboratory. However, currently available methods to enrich for regions of interest in the DNA sequence suffer from drawbacks such as high cost, complex protocols, lack of clinical-level accuracy and uneven target coverage. A target-enrichment approach using complementary long padlock probes described in a recent article significantly improves on previous methods in most of these areas. See related Research: http://genomemedicine.com/content/5/5/50.
AB - Targeted next-generation sequencing is becoming a common tool in the molecular diagnostic laboratory. However, currently available methods to enrich for regions of interest in the DNA sequence suffer from drawbacks such as high cost, complex protocols, lack of clinical-level accuracy and uneven target coverage. A target-enrichment approach using complementary long padlock probes described in a recent article significantly improves on previous methods in most of these areas. See related Research: http://genomemedicine.com/content/5/5/50.
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UR - http://www.scopus.com/inward/citedby.url?scp=84878005121&partnerID=8YFLogxK
U2 - 10.1186/gm450
DO - 10.1186/gm450
M3 - Article
AN - SCOPUS:84878005121
VL - 5
JO - Genome Medicine
JF - Genome Medicine
SN - 1756-994X
IS - 5
M1 - 46
ER -