Improved coverage and accuracy with strand-conserving sequence enrichment

Toumy Guettouche, Stephan L Zuchner

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Targeted next-generation sequencing is becoming a common tool in the molecular diagnostic laboratory. However, currently available methods to enrich for regions of interest in the DNA sequence suffer from drawbacks such as high cost, complex protocols, lack of clinical-level accuracy and uneven target coverage. A target-enrichment approach using complementary long padlock probes described in a recent article significantly improves on previous methods in most of these areas. See related Research: http://genomemedicine.com/content/5/5/50.

Original languageEnglish
Article number46
JournalGenome Medicine
Volume5
Issue number5
DOIs
StatePublished - May 29 2013

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Molecular Pathology
Clinical Protocols
Costs and Cost Analysis
Research

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Molecular Biology
  • Molecular Medicine

Cite this

Improved coverage and accuracy with strand-conserving sequence enrichment. / Guettouche, Toumy; Zuchner, Stephan L.

In: Genome Medicine, Vol. 5, No. 5, 46, 29.05.2013.

Research output: Contribution to journalArticle

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